the aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. in this experiment, different concentrations of glutathione [0 (lg-0), 0.5 (lg-0.5), 1 (lg-1), 1.5 (lg-1.5), and 2 (lg-2) mm] were added in a soybean lecithin-based extender. a total of 16 ejaculates from four bucks were collected and pooled. each pooled sample was divided into five...

BACKGROUND Variability among stallions in terms of semen cryopreservation quality renders it difficult to arrive at a standardized cryopreservation method. Different extenders and processing techniques (such us colloidal centrifugation) are used in order to optimize post-thaw sperm quality. Sperm chromatin integrity analysis is an effective tool for assessing such quality. The aim of the presen...

Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytom...

Egg yolk low-density lipoproteins (LDL) and soybean lecithin were evaluated as replacements for egg yolk in extenders used for the cryopreservation of brown-bear spermatozoa. The motility, viability and acrosomal status of post-thawed spermatozoa were analysed, and an egg-yolk extender was used as a control. The total antioxidant capacity of these extenders was tested. Soybean lecithin showed a...

Origanum vulgare contains high levels of phenolic compounds such as gallic acid and polyphenols such as rosmarinic acid and quercetin. The purpose of this study was to investigate the effects of Origanum vulgare extract, as a natural antioxidant, on freezing-thawing semen quality in Holstein bulls. Three Holstein bulls (5-6 years old, mean live weight 800 kg) were used for semen collection twic...

AIM To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. METHODS Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were forme...

In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of yolk is largely presumed to be due to low density lipoproteins (LDL). The effects of LDL on sperm quality of bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has been m...

The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total of 16 ejaculates from four bucks were collected and pooled. Each pooled sample was divided into five...

The aim of this study was to determine the effects of co-Q10 and ellagic acid on sperm parameters after the freeze–thawing of Ghezel ram sperm. Twenty ejaculates from five Ghezel rams were collected in this study. Semen samples, which were diluted with a soybean lecithin (SL) based extender containing 0.25 mM ellagic acid, 0.5 μM co-Q10, 0.25 mM ellagic acid + 0.5 μM co-Q10 and no antioxidant (...