in this paper, multi-wall carbon nanotubes (mwcnts), gold nanoparticles (gnp) and glucose oxidase (god) was developed for the specific detection of glucose. mwcnts were chemically modified with the h2so4–hno3 pretreatment to introduce carboxyl groups which were used to interact with the amino groups of poly(allylamine) (paa) and cysteamine via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/n-hy...

This paper reports a novel approach in the electrode immobilization of an enzyme, invertase, by electrochemical polymerization of pyrrole in the presence of enzyme. The polypyrrole/invertase and polyamide/polypyrrole/invertase electrodes were constructed by the entrapment of enzyme in conducting matrices during electrochemical polymerization of pyrrole. This study involves the preparation and c...

The fabrication of enzyme electrodes using self-assembled monolayers (SAMs) has attracted considerable interest because of the spatial control over the enzyme immobilization. A model system of glucose oxidase covalently bound to a gold electrode modified with a SAM of 3-mercaptopropionic acid was investigated with regard to the effect of fabrication variables such as the surface topography of t...

We present a rate-determination method for analyzing glucose. A glucose enzyme electrode serves as the sensor and is made by placing a gel-immobilized layer of glucose oxidase over the tip of a Clark-type O2 electrode. The electrode membrane is made of Teflon and is derivatized by etching with a suspension of colloidal sodium metal in organic solvent. The enzyme is coupled to the membrane surfa...

This study aims to characterize the tungsten-urea analyte indicator electrode. The method used is biosensor potentiometry with urease enzyme immobilization technique. electrode was coated PVA-enzyme glutaraldehyde (GA) 2.9% PVC-KTpClPB- o-NPOE variation of 61% and 66%. Characterization electrodes using SEM-EDS, FTIR XRD analysis. A1-4 sample 1x 1x, 61%. A3-4 3x Likewise, reasoning samples 66% T...

We fabricated a thermostable quinoprotein aldose sugar dehydrogenase (tPQQ-ASD derived from a hyperthermophilic archaeon Pyrobaculum aerophilum) immobilized electrode. The electrode was prepared by immobilizing agarose gel mixed with the enzyme and carbon nanofiber (CNF) on a carbon paste (CP) electrode containing p-benzoquinone (BQ) as an electron mediator. The electrocatalytic response was ...

Cells Sergey V. Morozov, Arkady A. Karyakin, Paulette M. Vignais, Serge Cosnier Faculty of Chemistry, M.V.Lomonosov Moscow State University, 119992, Moscow, Russia CEA/Grenoble, BBSI (UMR CEA/CNRS/UJF no. 5092), DRDC, 38054 Grenoble Cedex 9, France 3 LEOPR (UMR CNRS no. 5630), UJF, BP 53, 38041 Grenoble Cedex 9, France Molecular hydrogen is considered nowadays as the most promising chemical fue...

Enzyme modified electrodes were fabricated with poly(aniline-co-maminophenol). Electrochemical polymerization of aniline and m-aminophenol was performed to get the film of copolymer on the surface of gold electrode. Modified electrodes were fabricated by two methods, physical entrapment and covalent cross-linking. In one of the method, gold nanoparticles were loaded into the copolymer film and ...

In 1962, Clark and Lyons proposed incorporating the enzyme glucose oxidase in the construction of an electrochemical sensor for glucose in blood plasma. In their application, Clark and Lyons describe an electrode in which a membrane permeable to glucose traps a small volume of solution containing the enzyme adjacent to a pH electrode, and the presence of glucose is detected by the change in the...