Background: Silk fibroin is a suitable protein for osteogenesis by inducing markers of bone formation in the cultures of osteoblasts, so we examined the ability of this protein to induce mineralized nodules in the rat bone marrow stromal cell cultures. Methods: Bone marrow stromal cells obtained from 4 to 6 weeks old Spruge-Dawely male rats were grown in primary culture for seven days and then ...

background: bone marrow stromal cells (bmsc) are used as a source for cell therapy in different model for neurological disorder such as stroke and spinal cord injury. however, the transdifferentiation of bmsc into cholinergic phenotype requires more investigation. methods: bmsc were isolated from adult rats, pre-induced with β-mercaptoethanol (bme) and followed by nerve growth factor (ngf) indu...

abstractbackground: fetal bovine serum (fbs) is widely used in cell culture laboratories,risk of zoonotic infections and allergic side effects create obstacles for its use inclinical trials. therefore, an alternative supplement with proper inherent growthpromotingactivities is demanded.objective: to find fbs substitute, we tested human umbilical cord blood serum(hucs) for proliferation of human...

Hematopoietic stem/progenitor cells (HSPCs) which give rise to different blood cell types are present within the bone marrow microenvironment, especially in flat bones such as skull, vertebrae, pelvis and chest. Interacting factors such as stromal derived factor-1/CXCR4, very late antigen-4/vascular cell adhesion molecule-1, Lymphocyte function-associated antigen-1/ intercellular adhesion molec...

introduction: an induction of osteogenic differentiation in rat bone marrow stromal cell cultures by dexamethasone was reported. materials and methods: the marrow stromal cells obtained from 4 to 6 weeks - old spruge-dawely male rats were grown in primary culture for 7 days and then subcultured for 20 days. the cells were cultured in either dmem medium containing 15% fetal calf serum, antibioti...

bone marrow mesenchymal stem cells can differentiate into neurons and astrocytes after transplantation in the spinal cord injury of rats. although bone marrow mesenchymal stem cells are known to protect against spinal cord injury through anti-apoptotic effects, but generation of neural stem cells with large number and safety protocols unclear. in the present study, described the differentiation...

Bone marrow is the point of origin of several cell types, including stromal cells. Adherent bone marrow stromal cells can differentiate into chondrocytes, adipocytes and osteoblasts. Several substances which modulate the dynamics and differentiation of bone marrow stromal cells have been identified. Recently it has been discovered that those bone marrow cells which are non-adherent in tissue cu...