evidences from many studies suggested a polymerase chain reaction (pcr) as a valuable method for diagnosing infectious disease in the transplant recipients. we used this method for detection of toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. dna of both infectious agents were detected using two separate sets of nested pr...

Bovine viral diarrhoea virus (BVDV) is an important pathogen of dairy cattle. In this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of Mashhad- Iran, were tested for presence of BVDV by the use of a nested reverse transcription polymerase chain reaction (Nested RT- PCR) assay. Non of the cows in the herds had been vaccinated against...

Several methods for the detection of Borna disease virus (BDV) RNA have been reported, one being the reverse transcription-nested polymerase chain reaction (RT-nested PCR) method. However, due to the possibility of contamination of the cloned DNA in a reaction tube, false-positive results might be obtained by RT-nested PCR. To detect only BDV RNA without anxiety of contamination, we developed a...

A reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) was standardised to detect bovine respiratory syncytial virus (BRSV), using a Brazilian isolate, in three experimentally infected calves. This followed initial tests in infected chicken embryo related (CER) cells. One animal had lesions, characterized by interstitial multifocal pneumonia, severe interstitial and subpleural emph...

Mycoplasma gallisepticum is the most important and infectious Mycoplasmosis. It is caused lots of economic losses for poultry's industry of Iran. The target of this study is comparison of culture and nested PCR techniques to detect Mycoplasma gallisepticum infection of chicken’s from industrial farms in Kerman province of Iran. 88 isolates received from industrial far...

Human metapneumovirus (hMPV) is an etiologic agent of respiratory tract infections. In this study, we compared the sensitivity and specificity of real-time reverse transcription (RT)-polymerase chain reaction (PCR), conventional RT-PCR, and nested PCR in detecting hMPV genes. A total of 146 clinical specimens from 143 patients who showed acute respiratory tract infection symptoms were tested by...

A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...

Mycoplasma gallisepticum is the most important and infectious Mycoplasmosis. It is caused lots of economic losses for poultry's industry of Iran. The target of this study is comparison of culture and nested PCR techniques to detect Mycoplasma gallisepticum infection of chicken’s from industrial farms in Kerman province of Iran. 88 isolates received from industrial far...