Background: While assisted reproductive techniques (ARTs) are considered to be safe, many concerns exist about the safety of these techniques. Two important and indispensable parts of these techniques are in vitro culture and vitrification. While much progress has been made in developing culture conditions, it remains suboptimal. In order to investigate the effect of culture medium on in vitro ...

Serum-free culture is now becoming the general trend of biological production. Its application in Vero cell culture is significant. In the present paper, we reviewed factors affecting Vero cell serum-free culture and several culture techniques. In light of these reviews, we outlined its extensive application prospect.

The development of biomimetic microenvironments will improve cell culture techniques by enabling in vitro cell cultures that mimic in vivo behavior; however, experimental control over attachment, cellular position, or intercellular distances within such microenvironments remains challenging. We report here the rapid and controllable immobilization of suspended mammalian cells within microfabric...

background: chlamydia trachomatis is the most reported bacterial sexually transmitted disease, especially among young women worldwide. aim of this study was comparison to evaluate the prevalence of chlamydia trachomatis infection in woman with tubal infertility by means of pcr and cell culture techniques. methods: fifty-one women with confirmed tfi were enrolled in this study in (avicenna infer...

Mild hypothermia condition in mammalian cell culture technology has been one of the main focuses of research for the development of breeding strategies to maximize productivity of these production systems. Despite the large number of studies that show positive effects of mild hypothermia on specific productivity of r-proteins, no experimental approach has addressed the indirect effect of lower ...

Background: Mammalian Cell lines are the main expression system for the production of recombinant therapeutic proteins. Optimization of cell culture condition is performed via alteration in different parameter. Cell culture media plays an important role in cell cycle because of compounds such as amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, trace elem...

The protein-stabilizing and cell-penetrating activities of Bombyx mori 30Kc19 α-helix domain (30Kc19α) are investigated. Recently, 30Kc19 protein has been studied extensively as it has both protein-stabilizing and cell-penetrating properties. However, it is unknown which part of 30Kc19 is responsible for those properties. 30Kc19 protein is composed of two distinct domains, an α-helix N-terminal...