Background Morphine as a natural alkaloid (opiate) is considered as one of the problems associated with poor semen production and sperm quality. For the first time, this experimental study was carried out to evaluate the impact of intra-peritoneal injection of morphine on sperm parameters, chromatin quality and DNA integrity of spermatozoa aspirated from cauda epididymis of mice. MaterialsAndMe...

background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...

Received: 13 November 2010 Revised: 16 May 2011 Accepted: 15 June 2011 Abstract Background: Sperm cells extracted from testes (TESE) have poor chromatin quality and motility. Various substances are used in the laboratory to increase sperm motility and improve the ART outcomes; however, there are few research which considered improving both sperm motility and chromatin quality. Objective: The ai...

background: intrauterine insemination (iui) is one of the therapeutic approaches for infertility. the objective of this study was to evaluate dna integrity and apoptosis role in success of iui in both mild male and female factor infertility. methods: patients were divided into two groups: m (mild male factor; n=29) and f (female factor; n=31) undergoing single iui. ejaculates were analyzed and ...

Background: Alcohol abuse is considered as one of the problems associated with poor semen production and sperm quality. Both acute and chronic alcohol consumption may affect spermatozoal chromatin disorders through apoptosis. Therefore, for the first time, this experimental study was performed to evaluate the effect of ethanol consumption on sperm parameters and chromatin integrity of spermatoz...

Sperm chromatin integrity has been being recognized as an important factor in male fertility. During normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. However, using Assisted Reproductive Techniques, particularly ICSI, the natural selection is bypassed. Therefore sperm with DNA breakage have the opport...

Evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. Routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin struc...

introduction: date palm gemmule has been used in traditional medicine to improve the semen quality and treat infertility. their components remedies those are rich of antioxidant and vitamins can influence spermatogenesis. the aim of this project was to evaluate the effects of date palm gommule water/alcoholic extract on reproductive tissues, sex hormone and sperm quality of rat. methods: sevent...

Bone-marrow mesenchymal stem cell (BM-MSC) proliferation and lineage commitment are under the coordinated control of metabolism epigenetics; MSC niche contains low oxygen, which is an important determinant cellular metabolic state. In turn, drives fate decisions via alterations chromatin landscape. Due to fundamental role BM-MSCs in development adipose tissue, bones cartilage, age-associated ch...