نتایج جستجو برای: cryotop cryoprotectant embryo mouse oocyte vitrification
تعداد نتایج: 347340 فیلتر نتایج به سال:
Background: Embryo vitrification was effectively used for assisted reproductive techniques. Despite the undeniable benefits of vitrification, cooling and warming stress, and cytotoxicity of cryoprotectant may affect the DNA methylation that have an important role in gene activation and silencing. In the present study effects of 2-cell embryo vitrification on DNA methylation in hatched blastocys...
Gorgonian corals are slowly declining due to human interaction and environmental impacts. Cryopreservation of gorgonian corals is an ex-situ method of conservation, ensuring future reproduction. The present study assessed the vitrification properties of cryoprotectant (CPT) mixtures using the cryotop, cryoloop and open pulled straw (OPS) cryopereservation methods prior to experimentation on gor...
Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compar...
background: an evaluation of the developmental competence of vitrified mouse germinal vesicle (gv) oocytes with various equilibration and vitrification times; in the presence or absence of cumulus cells and by comparison between the cryotop method and straws was performed. materials and methods: mouse gv oocytes were considered in cumulus-denuded oocytes (cdos) and cumulus-oocyte complexes (coc...
STUDY QUESTION Can the equilibration steps prior to embryo vitrification be automated? SUMMARY ANSWER We have developed the 'Gavi' system which automatically performs equilibration steps before closed system vitrification on up to four embryos at a time and gives in vitro outcomes equivalent to the manual Cryotop method. WHAT IS KNOWN ALREADY Embryo cryopreservation is an essential componen...
Background: Vitrification has been usually used as an assisted reproductive technology in animals and humans. This method needs high concentrations of cryoprotectants that can be toxic with high cooling degrees. Then, vitrification could be change histone modifications such as methylation and acetylation can performance as regulatory controls of gene transcription. So, the purpose of the presen...
Comparison of Ethylene Glycol and Propylene Glycol for the Vitrification of Immature Porcine Oocytes
Our aim was to optimize a cryoprotectant treatment for vitrification of immature porcine cumulus-oocyte complexes (COCs). Immature COCs were vitrified either in 35% ethylene glycol (EG), 35% propylene glycol (PG) or a combination of 17.5% EG and 17.5% PG. After warming, the COCs were in vitro matured (IVM), and surviving oocytes were in vitro fertilized (IVF) and cultured. The mean survival rat...
The ability to cryopreserve human oocytes and store them indefinitely would be beneficial for cancer patients at risk of becoming sterile after therapy, allow women to delay reproduction, and alleviate religious concerns associated with embryo storage. In 1986, Chen was the first to report a pregnancy originating from a frozen–thawed human oocyte. Although over 100 babies have been born from oo...
Is there a really need to validate oocyte vitrification technique in an ART laboratory before establishing it in daily practice? Validation of micromanipulationbased technique, in this case oocyte vitrification, is essential prior to enlarging its use to routine practice. Oocyte vitrification is a new worldwide used technique and legal recently in France. This micromanipulation needs to be perf...
Background and purpose: To assess the effect of equilibration time on the vitrified-warmed two cell mouse embryos. Material and Methods: Two cell embryos were obtained from oviducts of super ovulated female NMRI mice. The embryos were randomly allocated in four groups and vitrified using cryotop and two-step mediaprotocol. The experimental groups were: 1) embryos were equilibrated in vitrif...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید