A polarographic technique suitable for kinetic studies of either isolated or bound pyridine nucleotide-linked dehydrogenases was used to investigate the isocitrate dehydrogenases of yeast mitochondria. Intact mitochondria were found to contain both triphosphopyridine nucleotideand diphosphopyridine nucleotide-linked isocitrate dehydrogenases with properties very similar to those which have been...

Antisera were prepared against pure nicotinamide adenine dinucleotide-dependent d-lactic dehydrogenases of Lactobacillus leichmannii, L. jensenii, and L. fermenti. When tested against these three antisera, crude extracts of almost all species of Lactobacillus containing a d-lactic dehydrogenase give cross-reactions. Extensive pairwise comparisons between cross-reacting crude extracts by double ...

During aerobic respiration, cells produce energy through oxidative phosphorylation, which includes a specialized group of multi-subunit complexes in the inner mitochondrial membrane known as the electron transport chain. However, this canonical pathway is branched into single polypeptide alternative routes in some fungi, plants, protists and bacteria. They confer metabolic plasticity, allowing ...

The distribution and localization-pattern of certain DPN-linked dehydrogenases (malic, lactic, D-glucose, glutamic, and a-glycerophosphate) were demonstrated histochemically in the red and white fibres of pigeon breast-muscle by using neotetrazolium as the hydrogen acceptor, under strictly anaerobic conditions. All the dehydrogenases studied showed distinctly higher enzyme activity in the narro...

The aim of the study was to determine the response of soil enzymes such as dehydrogenases, urease and acid and alkaline phosphatases to heating oil contaminating (0.0, 0.25, 0.5, 0.75, 1.0, 1.5% of soil) the experimental soil supplemented with lime and used for cultivation of yellow lupine of the Markiz variety. An increasing contamination of soil with heating oil stimulated the activity of deh...

Horikoshi, Koki (The Institute of Physical and Chemical Research, Tokyo, Japan), Shigeji Iida, and Yonosuke Ikeda. Mannitol and mannitol dehydrogenases in conidia of Aspergillus oryzae. J. Bacteriol. 89:326-330. 1965.-A sugar alcohol was isolated from the conidia of Aspergillus oryzae and identified as d-mannitol. Two types of d-mannitol dehydrogenases, nicotinamide adenine dinucleotide phospha...

accurate protein function prediction is an important subject in bioinformatics, especially wheresequentially and structurally similar proteins have different functions. malate dehydrogenaseand l-lactate dehydrogenase are two evolutionary related enzymes, which exist in a widevariety of organisms. these enzymes are sequentially and structurally similar and sharecommon active site residues, spati...

9. Doonan, S., Martini, F., Angelaccio, S., Pascarella, S., Barra, D. & Bossa, F. ( 1986) J. Moi. Evol. 23, 328-335 McAllister-Henn, L. ( 1988) Trends Biocherrr. .ki . 13, 1781 8 1 Doyle, J. M. (1988) Ph.D. Thesis, National University of Ireland Martini, F., Angelaccio, S., Barra, D., Pascarella, S., Maras, B., Doonan. S. & Bossa, F. (1985) Hiochirn. Hiophys. Actcr 832, 46-5 I Lipman. D. J . & ...

Cell-free extracts of Pseudomonas aeruginosa strains, grown on ethanol, showed dye-linked alcohol dehydrogenase activities. The enzyme responsible for this activity was purified to homogeneity. It appeared to contain two molecules of pyrroloquinoline quinone per enzyme molecule. In many respects, it resembled other quinoprotein alcohol dehydrogenases (EC 1.1.99.8), having a substrate specificit...