By employing the embryonic duck heart method to assay digitoxin quantitatively in extracts of human urine, it was found that, contrary to previous opinion, a large percentage of ingested or in-injected digitoxin is excreted via the kidney in a physiologically active state. Approximately 40 per cent of a digitalizing dose (1.2 mg.) is excreted over a period of 12 to 24 days. The average rate of ...

SUMMARY Despite recent advances in understanding of the pharmacokinetics and electrophysio-logic effects of cardiac glycosides, digitalis toxicity remains distressingly common in clinical practice. Another substantial group of patients is markedly underdigitalized, failing to gain the full therapeutic benefits of optimal use of these drugs. Since cardiac digitalis toxicity is a dose-related phe...

This fluoroimmunoassay for digitoxin in serum involves use of a sheep antiserum to digitoxin coupled to magnetizable solid-phase particles and fluorescein-labeled 3-O-succinyl digitoxigenin as tracer. Sodium salicylate blocks binding of the drug by binding proteins, and endogenous fluorophores and other interfering components in serum samples are reliably and completely removed at the separatio...

The role of the peritrophic envelope in the non-absorption of three allelochemicals ingested by generalist grasshoppers was examined. This study tested the hypothesis that the association of lipophilic and amphiphilic allelochemicals with lipid aggregates (mixed micelles) reduces their permeability through the peritrophic envelope, a process similar to extractive ultrafiltration. Each of three ...

Pharmazeutisches Institut der Universität, Auf der Morgenstelle 8, D-7400 Tübingen, Bundesrepublik Deutschland Z. Naturforsch. 43c, 199-206 (1988); received December 8, 1987 Cardiac Glycosides, Cytochrome P-450, Digitalis lanata, Digitoxin 12ß-Hydroxylase, Endoplasmic Reticulum The cytochrome P-450-dependent monooxygenase digitoxin 12ß-hydroxylase from cell cultures of Digitalis lanata needs NA...

OBJECTIVES Digitalis therapy (digoxin or digitoxin) in patients with heart failure is subject to an ongoing debate. Recent data suggest an increased mortality in patients receiving digitalis. This study investigated the effects of chronic digitalis therapy prior to heart transplantation (HTX) on posttransplant outcomes. PATIENTS AND METHODS This was a retrospective, observational, single-cent...

To determine the effect of prolonged digitoxin administration on contractile function of nonfailing myocardium, right ventricular papillary muscle mechanics were examined after 6 or 24 wk of glycoside administration to control and pulmonary artery banded cats. Resting length-tension relations were not affected by digitoxin; however, isometrically developed force and the maximal rate of force de...

The effects of chronic treatment with digitoxin on arterial baroreceptor sensitivity for heart rate (HR) and renal sympathetic nerve activity (rSNA) control, cardiopulmonary reflex, and autonomic HR control in an animal model of heart failure (HF) were evaluated. Wistar rats were treated with digitoxin, which was administered in their daily feed (1 mg/kg per day) for 60 days. The following 3 ex...

Rats given a parenteral injection of digitoxin (1 pg. per gram of body weight) were found to excrete approximately 15 to 18 per cent of the dose in their feces. This fecal digitoxin however came from the bile, and there was no evidence of any active secretion of the glycoside by the bowel wall itself. FOR MANY YEARS workers" 2 have commented on the high degree of insensitivity to the toxic effe...