An extension of the stoichiometric displacement (SD) model for the ion-exchange adsorption of dilute proteins is developed which accounts for the effects of hydrogen ion Donnan equilibrium on the protein charge. The ability of the new model to fit retention data when the fluid phase pH is near the protein pI and the effects of hydrogen ion Donnan equilibrium are important is examined using four...

Membranes from stably transfected cell lines that express two point mutations of the human organic cation transporter-1 (hOCT1), R488M and G465R, have been immobilized on the immobilized artificial membrane (IAM) liquid chromatographic stationary phase to form two cellular membrane affinity chromatography (CMAC) columns, CMAC(hOCT1(G465R)) and CMAC(hOCT1(R488M)). Columns were created using both...

We have succeeded in isolating virus from the particle laden solution like body fluid. Because many of virus infectious diseases occur in the developing region, compact and low-cost device for virus detection from the patients’ body fluid is required. We proposed a microfluidic chip for virus isolation by hydroxyapatite chromatography. This microfluidic chip utilized a column and a pair of swit...

We assessed a simple method for purifying 125I-labeled human prolactin, taking advantage of the abundant supplies of monoclonal antibodies available. 125I-Labeled human prolactin purified by immunoaffinity chromatography is compared with that purified by gel filtration on Sephadex G-100. We used monoclonal antibodies to prolactin to prepare the affinity chromatography columns. Prolactin was rad...

Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. This method is based on the use of a biologically related agent as a stationary phase to selectively retain analytes or to study biological interactions. This review discusses the basic principles behind affinity chromatography and examines recent d...

The effect of isotherm parameters of a displacer on the efficiency of desorption Chromatography has been investigated numerically. A general nonlinear multicomponent rate equation model with Langmuir isotherm was used in this study. It was found that the best displacer in this kind of operation is usually not the one that is more strongly adsorbed than the adsorbates when the operation is to di...