objective: ferula szowitsiana has been widely used for medicinal purposes around the world. the anti-oxidant effect of f.szowitsiana had been proved. the current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from f. szowitsiana, against hydrogen peroxide (h2o2) - induced oxidative dna damage in human lymphocytes. materials and methods: human lymphocyt...

background: ˝radioadaptive response˝ is well-documented phenomenon appeared in low dose ionizing radiation received in vitro and in vivo. occupational exposure has always been a great concern for radiation workers therefore this study was performed to study radioadaptive response in terms of residual dna double strand breaks as an endpoint in peripheral blood leukocytes of occupationally expose...

background: exposure to ionizing radiation results in genotoxicity and the unrepaired lesions in cellular dna results in cell cycle arrest, reproductive death, interphase death, division delay, chromosome aberrations, mutations, etc. leading to the intensive destruction of cells and violation of their proliferative capacity there by adversely affecting the mammalian system. since ionizing radia...

ilex spinigera (aquifoliaceae) plant is an evergreen tree or shrub with thick glossy dark green leaves and red fruits. this plant has medicinal properties and has been used traditionally in northern iran for malaria treatment. the aim of this work is to evaluate the antioxidative activities and the inhibitory effect of i.spinigera on the oxidation of dna. we have found no reports about the popu...

abstract accumulation of gene changes and chromosomal instability in response to cellular dna damage lead to cancer. dna damage induces cell cycle checkpoints pathways. checkpoints regulate dna replication and cell cycle progression, chromatin restructuring, and apoptosis. checkpoint kinase 2 (chk2) is activated in response to dna lesions. atm phosphorylate chk2. the activated chk2 kinase can p...

this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...

background: sperm dna damage is an important factor in the etiology of male infertility. objective: the aim of the study was to evaluate the association of oxidative stress induced sperm dna damage with the pathogenesis of male infertility. materials and methods: the study comprised a total of 66 subjects, including fertile men (n=25) and infertile men (n=41) matched by age. seminal malondialde...

background: exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and dna through radiolysis of cellular water. since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the dna damage and repair kinetics would help to predict the radiation sensitivity of cel...