conclusions the results of this study demonstrated that the tpasp-padre-truncated orf2 gene cassette and the truncated orf2 gene in recombinant plasmids are successfully expressed in eukaryotic cells. the immunogenicity of the two recombinant plasmids with different formulations will be evaluated as a novel dna vaccine in future investigations. results sequence analysis and bioinformatics studi...

Sequence variants of human mitochondrial DNA (mt DNA) have been implicated in a variety disorders and conditions. Massive parallel sequencing is becoming increasingly popular due to its efficiency cost-effectiveness. In relation acquiring significant sequence information like levels heteroplasmy mt DNA, it offers marked improvement compared previous methods used. Here we describe variant callin...

  Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...

background : strongyloides stercoralis is a prevalent parasite in some rural areas in the north of iran. we decided to investigate whether the 18s ribosomal dna sequence of the parasite in iran is similar to the findings of the other re­searchers. methods :  we collected 3514 stool samples from gilan and mazandaran, northern iran, during the year 2005-2006, from which 96 were found infected wit...