In this study, a novel Escherichia coli-specific bacteriophage, vB_EcoM_IME392, was isolated from chicken farm sewage in Qingdao, China. The genome of IME392 found by next-generation sequencing to be 116,460 base pairs length with G+C content 45.4% (GenBank accession number MH719082). BLASTn results revealed that only 2% the sequence shows similarity known phage sequences GenBank database, whic...

in this study, 90 internal organ samples of poultry with symptoms of colibacillosis were obtained from maragheh poultry farms in east azerbaijan, iran. in total, 70 bacterial isolates were confirmed as escherichia coli (e. coli) strains using standard biochemical tests, and antibiotic sensitivity was determined by the disk diffusion method. antibiotics used in this study included ampicillin, pe...

A study was conducted to determine if prior exposure with bacteriophage would limit the ability of the same bacteriophage to treat colibacillosis. There were 5 treatments with 3 replicate pens of 20 birds per pen. The treatments consisted of 1) control, 2) birds treated with bacteriophage at 10 and 17 d of age, 3) birds challenged with Escherichia coli at 17 d of age, 4) birds challenged with E...

Bovine mastitis caused by Klebsiella pneumoniae is usually treated with antibiotics, thereby potentially increasing antimicrobial resistance. The objective of this study was to evaluate efficacy a bacteriophage, isolated from dairy farm wastewater, as treatment for murine model K. mastitis. A lytic bacteriophage CM8–1 isolated, morphological and biological characteristics were assessed transmis...

Combining genetic, biochemical and computational approaches, we elucidated the molecular mechanisms underlying recognition of Listeria wall teichoic acid by bacteriophage-encoded SH3b repeats.

Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-G...

The elegant geometry of viruses has inspired bio-engineers to synthetically explore the self-assembly polyhedral capsids employed protect new cargo or change an enzymatic microenvironment. Recently, Yang and co-workers used DNA nanotechnology revisit icosahedral capsid structure phiX174 bacteriophage reloaded original viral genome as into their fully synthetic architecture. Surprisingly, when u...

results the bacteriophage, designated mj1, was isolated by testing against a clinical mdr e. coli 3 strain. the mj1 phage showed a wide range of heat and ph stability. the phage morphology, determined by transmission electron microscopy, revealed a structure comprised of a head (108 ± 0.2 nm long by 128 ± 0.5 nm wide) and a contractile tail (123 ± 0.5 nm long by 15 - 26 nm wide). these features...