Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...

objective: vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. however, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. this study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mo...

objective(s) quinazolinones are heterocyclic components (able to form cyclized compounds) which have several medical effects such as anti-malarial, spasmolytic, anti-microbial, sedative, etc. they are also known for their fungicidal properties, inhibition of tyrosine-kinase and dna repair enzyme poly (adp-ribose) polymerase (parp) and are also effective in treatment of cancer, diabetes, and par...

for the first time, a spectrum of morphological abnormalities have been produced in developingmouse embryos (n=50), by injecting (ip) 75 and 100 mg/kg of maternal body weight of a new quinazolinonederivative, 4(3h)quinazolinone-2-propyl-2-phenylethyl(qppe), on days 9, 10 and 11 of gestation.measurements of the length of the crown-rump (cr), weight of body of the embryos and placenta on day 17sh...

background: matrigel (extracellular matrix) can improve the growth of many cell types in vitro. objective: the aim of the present study was to determine the effect of matrigel on the development of 2-4 cells human embryos in culture. material and methods: surplus 2-4 cells human embryos, resulting from icsi, were divided into two groups (control and test). quality of embryos in both groups was ...

Background: While it is possible to routinely cryopreserve embryos from several mammalian species, the cryopreservation of embryos has largely been limited by their high sensitivity to chilling injury. Many factors such as the stage of embryonic development, cryoprotectant toxicity, the composition of the vitrification solution and cooling and warming rates can influence survival of embryos aft...

background: the mitochondria are an important source of adenosine triphosphate (atp) production in pre-implantation embryo. therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and atp content. methods: the embryos at 2-pn, 4-cell and blastocyst stages were collected from the oviduct of st...

Background: Success in assisted reproductive techniques is raising hope for many infertile couples, but as in these techniques many embryos are made, there is no choice other than cryopreservation of the not-transferred embryos. Along with development of these techniques, the number of In-vitro made embryos is rising and the number of transferred embryos is decreasing to prevent multiple gestat...