objective(s) some properties of neuraminidase produced by pseudomonas aeruginosa pao1 growth in a defined medium (bhi) were examined and evaluated for its features. materials and methods the obtained supernatant enzyme of p. aeruginosa pao1 cultures was used in a sensitive fluorometric assay by using 2'-(4-methylumbelliferyl) a-d-n acetylneuraminic acid as substrate. as hydrolyzing mun wit...

a total number of  5520 female and  480 male breeders (arbor acres plus) at 42wks of age  were used in a 10-week-trial to investigate the effect of an enzyme complex on  the quality  and quantity of egg production as well as hatchability of broiler breeders fed a corn-soybean meal diet. there were two dietary treatment groups: a control group fed on a corn-soybean based diet, and the multi-enzy...

Background: Enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme β-galactosidase (β-gal, β-D-galactoside galactohydrolase, EC 3.2.1.23), trivial called lactase. Orthogonal arrays design is an appropriate option for the optimization of biotechnological processes for the production of microbial...

The objective of the present experiment was to quantify the gas production parameters, methane emission and microbial nitrogen production of a conventional total mixed ration including corn silage, alfalfa hay, wheat straw and concentrate (390, 110, 22 and 478 g/kg of diet DM, respectively) treated with different levels of Natuzyme® and pre-incubation time after enzyme administration. Two hundr...

A 3 × 2 factorial arrangements with completely randomized design, with a total of 72 Leghorn Hy-line (W-36) laying hens in the second cycle (98 wk old) of production were randomly assigned to 24 wire cages (45.7×30×30 cm3) and fed with diets containing three levels of guar meal (GM; 0, 4 and 8%) and two levels of β-mannanase (Hemicell®) enzyme (0.00 and 0.05%) for 12 wk period. There...

The aim of this study was production and partial purification of α-amylase enzyme by Bacillus licheniformis. B. Licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. Optimal conditions for amylase production by B. Licheniformis are incubation period of 120 h, temperature of 37 °C and pH 7.0. The α-amylase enzyme was purified by ion exchange chromatography ...

the aim of this study was production and partial purification of α-amylase enzyme by bacillus licheniformis. b. licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. optimal conditions for amylase production by b. licheniformis are incubation period of 120 h, temperature of 37 °c and ph 7.0. the α-amylase enzyme was purified by ion exchange chromatography ...

background and objective: glucoamylase is a potent starch degrading enzyme whose cheap production has been an area of research. its production by aspergillus niger in solid-state fermentation was studied using dried garden pea peel as a substrate, which enormously reduced the production cost. the current study intended to produce glucoamylase by a cost-effective strategy and exhaustively charac...