BACKGROUND The sensitivity and specificity of two in-house MAC-ELISA assays were tested and compared with the performance of commercially-available CTK lateral flow rapid test and EUROIMMUN IFA assays for the detection of anti-Chikungunya virus (CHIKV) IgM. Each MAC-ELISA assay used a whole virus-based antigen derived from genetically distinct CHIKV strains involved in two chikungunya disease o...

Serologic evaluation for Zika virus (ZIKV) infection currently includes an initial screen using an anti-ZIKV IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) followed by supplemental testing of specimens with nonnegative results by a plaque reduction neutralization test (PRNT). We compared the performance characteristics of three ELISAs for the detection of IgM class antibodie...

Autoantibodies may play an important role in pathogenetic mechanism of autoimmune diseases and cancer and could lead to the identification of novel prognostic and/or predictive biomarkers for disease management. The purpose of the study was to investigate the prevalence of antinuclear antibodies (ANA) in sera of the patients with autoimmune rheumatic disease and various cancer types. Sera were ...

In this study, the optimal combination of three commercial glycoprotein G-2 (gG-2)-based herpes simplex virus type 2 (HSV-2) type-specific enzyme-linked immunosorbent assays (Euroimmun anti-HSV-2 immunoglobulin G [IgG] ELISA [Eu2], Gull HSV-2-specific IgG ELISA [Gu2], and Radim HSV-2 IgG ELISA [Ra2]) and one gG-2-based HSV-2-specific immunoblot (Euroimmun anti-HSV-1/HSV-2 gG Western blot [EuW])...

Study objective: SARS-CoV-2, which causes coronavirus disease (COVID-19), continues to cause significant morbidity and mortality. The diagnosis of acute infection relies on reverse transcription-polymerase chain reaction (RT-PCR)-based viral detection. objective this study was evaluate the optimal serological testing strategy for anti-SARS-CoV-2 antibodies provides an important indicator prior ...

Background: There is limited knowledge on the potential applicability of fecal calprotectin (f-CP) as an inflammatory screening parameter in infantile colic (IC). This study aimed to evaluate f-CP in neonates with IC as a useful diagnostic indicator regarding this condition. Methods: The present study was conducted on 100 cases, including 50 newborns with IC and 50 non-colicky neonates. The dia...

The presence of antibodies against endemic coronaviruses has been linked to disease severity after SARS-CoV-2 infection. Assays capable concomitantly detecting coronaviridae such as OC43, 229E, NL63, and may help elucidate this question. We developed a serum screening platform using bead-based Western blot system called DigiWest, running hundreds assays microgram amounts protein prepared direct...