results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...

the ability of tnf related apoptosis inducing ligand/apo2 ligand (trail/apo2l) in order to selectively induce apoptosis in tumor cells but not normal cells makes it an attractive target for development of new cancer therapy. although trail/apo2l has been produced in several hosts, e. coli is one of the best expression systems among them due to its safety, simplicity, low cultivation cost, and k...

tumor necrosis factor-related apoptosis-inducing ligand (trail), a member of tnf family, is an interesting ligand which selectively induces apoptosis in tumor cells and, therefore, it has been developed for cancer therapy. this ligand has been produced by various hosts such as e.coli. however, protein expression in e.coli cytoplasm leads to problems such as incorrect folding, reduction in biolo...

Elaboration of different toxins by enterotoxigenic E. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. Various strategies have been employed to raise antibodies against these toxins as a line of defense. In this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...

coq10 and lycopene are isoprenoid compounds with nutraceutical and pharmaceutical benefits. in this study, the effect of concomitant lycopene biosynthesis on coq10 accumulation in transformed escherichia coli dh5α was studied. a lycopene production pathway including geranylgeranyl diphosphate synthase (crte), phytoene synthase (crtb), and phytoene desaturase (crti) from erwinia herbicola was co...

introduction: hepatitis c virus (hcv) infection is a major public health problem which influences about 170 million people worldwide. different types of vaccines have been designed using hcv structural proteins to control the viral infection. the core nucleocapsid protein is one of the most conserved proteins and a desirable target for hcv vaccines, especially the protein-based vaccines. in cur...

human insulin-like growth factor 1 (higf-1) consists of 70 amino acids in a single chain with three intermolecular disulfide bridges possessing valuable therapeutic effects. to date, numerous variants of specifically engineered higf-1 have been produced so as to improve higf-1 biological activity, stability and stronger binding to igf-1 receptor. mecasermin is one of the modified variants with ...

avian pathogenic e. coli (apec) is responsible for economic losses in all poultry farms. certain virulence factors have been proposed as a means of controlling apec infections, including some proteins to be used for vaccination. in the study we report here, one of the major virulence factors, the iss (increased serum survival) gene, from e. coli strain χ1378, isolated from poultry colibacillosi...