Background: Failed fertilization after intracytoplasmic sperm injection (ICSI) can be due to a reduced oocyte-activation capacity caused by reduced concentrations and abnormal localization of the oocyte-activation factor phospholipase C (PLC) zeta. Patients with this condition can be helped to conceive by artificial activation of oocytes after ICSI with calcium ionophore (assisted oocyte activa...

BACKGROUND Failed fertilization occurs in 2-3% of ICSI cycles and is mainly due to lack of oocyte activation. Heterologous ICSI of patient's sperm in mouse oocytes allows discrimination between sperm- and oocyte-related aetiologies of activation failure. Assisted oocyte activation (AOA) by Ca-ionophore treatment can initiate fertilization in subsequent therapeutic ICSI. We report on diagnosis a...

background: the high fertilization failure after ivf treatment cycles could be related to chromosomal abnormalities. this study was carried out to assess the frequency of chromosomal abnormality on human oocytes lacking signs of fertilization 18-20 h after insemination . materials and methods: on day one, 18-20 h after insemination (ivf), fertilization was confirmed when two pronuclei (normal i...

Fertilization involves a series of cellular interactions culminating in the fusion of gamete membranes, creating a zygote and then an embryo. During the process of human fertilization in vivo or in conventional in vitro fertilization (IVF), sperm must be capable of undergoing the acrosome reaction, binding to the zona pellucida (ZP), and penetrating the ZP to fuse with the oolema. The key role ...

The aim of this report has been evaluating protamine content and DNA integrity of two patients with globozoospermia undergoing Intra Cytoplasmic Sperm Injection (ICSI). Semen analysis was carried out according to WHO criteria. Protamine deficiency and DNA fragmentation was assessed using Chromomycin A3 and sperm chromatin dispersion assay respectively. ICSI and chemical activation were carried ...

Background Our objective was to evaluate the effectiveness of clomiphene citrate (CC) vs. letrozole (L) plus human menopausal gonadotropin (hMG) in gonadotropin releasing hormone (GnRH) antagonist protocol in poor prognosis women with previous failed ovarian stimulation undergoing intracytoplasmic sperm injection (ICSI). MaterialsAndMethods This retrospective cohort study included cycles with C...

It is imperative to understand the molecular basis of various steps involved during fertilization. In the manuscript by Bianchi et al. a novel protein, Juno on egg membrane (oolemma) has been characterized that binds to sperm specific protein, Izumo-1. Monoclonal antibodies against Juno inhibited in vitro fertilization. Juno knock-out female mice failed to deliver litters on mating. It is rapid...

The latest micromanipulative technique, intracytoplasmic sperm injection (ICSI), has been very successful in cases of severe male factor infertility. The main objective of this study is to report the results of our fIrst experience with ICSI in cases of male factor infertility, as well as patients who had failed their previous cycles with conventional in vitro fertilization (IVF). Normal ...

background: recently, motile sperm organelle morphology examination (msome) criteria as a new real time tool for evaluation of spermatozoa in intracytoplasmic sperm injection (icsi) cycles has been considered. objective: the aim was to investigate the predictive value of msome in in vitro fertilization (ivf) in comparison to icsi cycles and evaluation of the association between msome parameters...