Background: In reverse genetics studies, minigenomes of negative sense RNA viruses are used to model the process virus replication and evaluate rescue system in course construction recombinant virus. current study, a minigenome (MG) for lentogenic D58 vaccine strain Avian orthoavulavirus 1 (AOAV-1) (Newcastle disease virus) was developed establish genetic this strain. Methods: The constructed b...

BACKGROUND Metagenomics can provide important insight into microbial communities. However, assembling metagenomic datasets has proven to be computationally challenging. Current methods often assemble only fragmented partial genes. RESULTS We present a novel method for targeting assembly of specific protein-coding genes. This method combines a de Bruijn graph, as used in standard assembly appr...

Circadian clocks are auto-regulatory loops in which 'clock' gene products feedback to regulate their own expression. This explains how they keep oscillating, but how do they first get going? It appears that the transcription factor dClock not only drives the oscillation within the fruit fly's clock, but also plays a pivotal role in pre-assembling the clockwork.

We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thali...

Abstract Purpose Gene therapy is a promising and novel therapeutic strategy for many mutated gene-associated diseases, including breast cancer. However, it poses significant biological drawbacks such as rapid clearance from the circulatory system low cellular uptake of exogenously delivered functional nucleic acids. The development efficient biocompatible carriers genetic materials has been ext...

an interface part is used between clutch pedal and clutch cable in peugeot, samand, rd and peugeot pars. this part is consisted of two pieces. the main body is made of polyamide 6.6 and the second part is made of ck45.due to the fact that the two pieces are made of different assembling geometry with respect to each other, therefore, has caused performance problems which may result in clutch cab...

Nowadays, tube bonding in vehicles structure is noticeably considered by many researches. In this paper, local tube compression process by a rubber ring has been studied. Firstly, simulation of the process was performed by FEM. Then, experimental test has been carried out to validate the numerical results. Numerical and experimental results were compared showing a good agreement. In the second ...