Rat epididymal fat pads were incubated in vitro at 37” in bicarbonate buffer, pH 7.4, with uniformly labeled pyruvateW. The net gas exchange, total CO:, production, O2 consumption, pyruvate utilization, lactate and glycerol production, and radioactive carbon incorporation into COZ, fatty acids, and glyceride glycerol were measured. The results were used to estimate the quantitative flow of pyru...

The occurrence and subcellular distribution of enzymes of the cytidine diphosphate choline pathway of lecithin synthesis have been examined. Choline kinase (EC 2.7.1.32) was completely soluble, while phosphorylcholine-cytidyl transferase (EC 2.7.7.15) and phosphorylcholine-glyceride transferase (EC 2.7.8.2) were associated with particulate fractions. Although components sedimenting at 10,000 to...

The glyceride ester derivatives 6a and 6b were prepared by reacting 1,2,3-trihydroxy propane 1,3-dipalmitate/stearate with (S)-naproxen as potential prodrugs. The synthesis was achieved successfully with the aid of N,N’-dicyclohexylcarbodiimide. These prodrugs were evaluated for anti inflammatory, analgesic and gastroprotective activity. It was found that prodrugs 6a and 6b showed less irritati...

In work previously reported from this laboratory it was found that CWlabeled glycerol, completely hydrolyzed from glycerides during digestion, was not utilized for the resynthesis of lymph glycerides (1). This suggests that glycerol per se may not be the precursor of glyceride glycerol. Since absorbed fatty acids appear in the lymph as triglycerides, it is necessary to account for the origin of...

Three pathways for phosphatidylcholine synthesis were assayed in castor bean (Ricinus communis var. Hale) endosperm. Phosphatidylethanolamine: S-adenosylmethionine methyl transferase occurred predominantly in the endoplasmic reticulum fraction, but some activity appeared in the mitochondria. Phosphorylcholine glyceride transferase occurred exclusively in the endoplasmic reticulum. The phosphory...

Three isoforms of dihydroxyacetone phosphate reductase in extracts from Dunaliella tertiolecta have been separated by a diethylaminoethyl cellulose column chromatography with a shallow NaCl gradient. The chloroplasts contained the two major isoforms, and the third, minor form was in the cytosol. The isoforms are unstable in the absence of glycerol and they are cold labile, but they may be parti...

GOODRIDGE, ALAN G., AND ERIC G. BALL. L$oganesis in the pigeon: in vivo studies. Am. J, Physiol. 2 I 3 (I) : 2 45-249. x g67,-Glucose-W4C was injected into fasted-refed pigeons and the amount of 14C incorporated into the fatty acids of liver, adipose tissue, and blood was determined at intervals thereafter up to 2 hr. Blood glucose and its radioactivity were monitored throughout the experiment....

A simplified method for the analysis of both the glyceride-rich Sf 20-1 O5 and the cholesterol-rich St 0-20 lowdensity lipoproteins is presented. It consists of serum lipoprotein fractionation by preparative ultracentrifugation and subsequent quantitative analysis by refractometry. Comparison of this technique with the technically more difficult analytic ultracentrifugal methodology reveals com...

A simple two-step procedure is now available for directly measuring triglycerides in whole serum and in lipoprotein fractions. I tested the performance of the assay, using pooled serum stored frozen, freshly prepared lipoprotein fractions, and pure glyceride standards. A parabolic concentration-response curve ensured linearity well beyond total glycerol concentrations of 10 mmol/L, but gave ris...

A simplified method for the analysis of both the glyceride-rich Sf 20-1 O5 and the cholesterol-rich St 0-20 lowdensity lipoproteins is presented. It consists of serum lipoprotein fractionation by preparative ultracentrifugation and subsequent quantitative analysis by refractometry. Comparison of this technique with the technically more difficult analytic ultracentrifugal methodology reveals com...