Poly-N-acetyllactosamine (PL) sequences (repeating (3Gal beta 1-4GlcNAc beta 1)n) in complex-type N-linked oligosaccharides often occur in branched tri- and tetraantennary chains containing alpha-linked mannosyl residues disubstituted by N-acetyllactosaminyl units at C-2 and C-6 (2,6-branched mannose). We report here our studies on the factors affecting PL biosynthesis and the branching of N-li...

Glycoproteomics recently became a very active field, mostly in mammals. The first part of this paper consists of a mini-review on the strategies used in glycoproteomics, namely methods for enrichment in glycoproteins and mass spectrometry (MS) techniques currently used. In a second part, these strategies are applied to the cell wall glycoproteome of etiolated hypocotyls of Arabidopsis thaliana,...

Identification of the cell surface glycoproteins is an essential requirement for elucidating the mechanisms that mediate intercellular adhesion and cell migration in gastrulating amphibian embryos. In our experiments the glycoproteins present at the surface of isolated cells at blastula and gastrula stages were labelled with 3H-labelled sodium borohydride. The procedure included the oxidation o...

ABSTRACT The movement of ciliary membrane proteins is directed by transient interactions with intraflagellar transport (IFT) trains. green alga Chlamydomonas has adapted this process for gliding motility, using retrograde IFT motors to move adhesive glycoproteins in the flagella membrane. Ca2+ signalling contributes directly process, although uncertainty remains over mechanism through which it ...

Glycoproteins are becoming popular in the pharmaceutical industry, prompting need for an effective spectroscopic technique that can differentiate them. SERS is one such ideal glycan analysis several key reasons, which discussed here.