Objective(s): The purpose of this study was investigation of the protein corona formation on the surface of zwitterionic nanoparticles when they exposed to bio-fluid like human plasma.Methods: Silica nanoparticles with zwitterionic surface coating, cysteine and sulfobetaine were employed as zwitterionic ligands, were synthesized and characterized in terms of physicochemical properties. To...

gabapentin is an anticonvulsant widely used in the treatment of epilepsy. no peculiar chromophore is available on the gabapentin moiety for direct analysis by absorption spectrophotometry. a sensitive spectrophotometric method for the determination of gabapentin in bulk, pharmaceutical formulations and human plasma has been developed. in this method, gabapentin directly derivatized with vanilli...

high-performance liquid chromatography (hplc) methods employing ultraviolet (uv) detector are not sufficiently sensitive to measure the low plasma concentrations following single oral dose of imipramine. therefore, in the present study a simple, rapid and yet sensitive hplc method with uv detection was developed and validated for quantitation of imipramine in human plasma samples. an efficient ...

the total vitamin c content in human plasma is widely accepted as an indicator of the tissue status of vitamin c. a liquid chromatography method with ultraviolet detector (264 nm) for measuring ascorbic acid in human plasma was developed. a c18 reversed-phase column and cetrimide as an ion-pairing agent was employed. ascorbic acid (aa) was measured after reducing l-dehydroascorbic acid to l-asc...

Gabapentin is an anticonvulsant widely used in the treatment of epilepsy. No peculiar chromophore is available on the gabapentin moiety for direct analysis by absorption spectrophotometry. A sensitive spectrophotometric method for the determination of gabapentin in bulk, pharmaceutical formulations and human plasma has been developed. In this method, gabapentin directly derivatized with vanilli...

Gabapentin is an anticonvulsant widely used in the treatment of epilepsy. No peculiar chromophore is available on the gabapentin moiety for direct analysis by absorption spectrophotometry. A sensitive spectrophotometric method for the determination of gabapentin in bulk, pharmaceutical formulations and human plasma has been developed. In this method, gabapentin directly derivatized with vanilli...

Background: In this study we have prepared the IgA solution from normal human plasma using plasma fractionation and ion exchange chromatography. Materials and Methods: Using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction III was prepared as a suitable source for IgA preparation. Then it was treated with caprylic acid for separation of impurities. For enrichm...

background: curcumin has shown to affect sperm motility and function in vitro and fertility in vivo. the molecular mechanism(s) by which curcumin affects sperm motility has not been delineated. since modulation of intracellular ph (phi) and plasma membrane polarization is involved in sperm motility, the present study was conducted to investigate the effect of curcumin on these sperm (human and ...

        A simple, reproducible and rapid gas chromatographic method for precise determination of valproic acid (VPA) in human plasma has been developed. Total time for sample preparation and GC analysis is less than 45 min. After plasma protein precipitation, VPA was extracted into chloroform with suitable recovery. By using Stabilwax®<span styl...

In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. Separation was achieved on a Zorbax...