We present a Transfer Learning (TL) framework based on Stacked Denoising Autoencoder (SDA) for the recognition of immunogold particles. These particles are part of a high-resolution method for the selective localization of biological molecules at the subcellular level only visible through Transmission Electron Microscopy (TEM). Four new datasets were acquired encompassing several thousands of i...

Scanning electron microscopes are useful biological tools that can be used to image the surface of whole organisms, tissues, cells, cellular components and macromolecules. Processes and structures that exist at surfaces can be imaged in pseudo or real 3D at magnifications of anything from about x10 to x1,000,000. Therefore a whole multicellular organism, such as a fly, or a single protein embed...

Our previous study has demonstrated intraneuronal localization of Nogo-A in motoneurons of the adult rat spinal cord. In the present study, we wished to explore Nogo-A immunoreactivity at synapses using pre-embedding and post-embedding immunogold cytochemistry. Immunogold particles for Nogo-A could be identified in dendrites of the spinal cord motoneurons. Some Nogo-A immunogold particles were ...

The inhibitory amino acid gamma-aminobutyric acid (GABA) is the major neurotransmitter in the globus pallidus. Although electrophysiological studies have indicated that functional GABA(B) receptors exist in rat globus pallidus, the subcellular localization of GABA(B) receptor subunits and their spatial relationship to glutamatergic and GABAergic synapses are unknown. Here, we use pre-embedding ...

The inherent surface charges and small diameters that confer colloidal stability to gold particle conjugates (immunogold) are detrimental to rapid cell surface labeling and distinct cluster definition in flow cytometric light scatter assays. Although the inherent immunogold surface charge prevents self aggregation when stored in liquid suspension, it also slows binding to cells to timeframes of...

background: pinopodes are suggested as biological markers of uterine receptivity, but their molecular components are unknown. objective: co-expression of galectin-3 and ?v?3 integrin at human pinopodes has been examined in this study to propose a role for them during adhesion phase of embryo implantation. materials and methods: biopsies were obtained from early and mid luteal phase endometrium ...