Biodegradation of keratin containing wastes viz. bird feathers, nails, hairs, animalwool is a low-cost, nutrient-rich biotechnological process that turns this plentiful waste into substance. It sustainable green approach towards the solution environmental threats created by these wastes. This study aimed to screen for potential keratinase-producing microorganisms and optimize physicochemical pa...

Based on previous screening for keratinolytic nonpathogenic fungi, Paecilomyces marquandii and Doratomyces microsporus were selected for production of potent keratinases. The enzymes were purified and their main biochemical characteristics were determined (molecular masses, optimal temperature and pH for keratinolytic activity, N-terminal amino acid sequences). Studies of substrate specificity ...

s of papers 25 and β-glucanase, except a low level of α-amylase activity (85,000 U/gm vs. 19,000,000 U/gm pure α-amylase). Results of chick growth at 21 d showed positive (P < 0.05) main effects of CP level and enzyme supplementation on BW and FCR. Birds receiving diets with higher CP content had higher BW (800 gm at 23% vs. 722 gm at 17% CP, P < 0.05). Birds fed diets supplemented with keratin...

the aim of this study was to find the optimal conditions of a newly isolated streptomyces strain jrs 19. screening for keratinase producing bacteria and their keratinase production were investigated. the keratinase produced by the isolate was purified by chromatographic techniques and the optimum ph maintained was 7.0 and maintained at different temperatures viz., 20ºc, 25ºc, 30ºc, 35ºc and 40º...

The present review deals with fungal keratinases including that of dermatophytes. Bacterial keratinases were also included. Temperature and substrate relationship keratinase production has also been discussed. Keratin degradation and industrial involvement of keratinase producing fungi is also reviewed.

Two experiments were conducted to investigate the in vitro ability of keratinase to hydrolyze soybean glycinin and β-conglycinin and to evaluate the in vivo effects of keratinase when included in corn-soybean diets with different levels of crude protein and fed to nursery pigs. In experiment 1, a saturated keratinase solution (1 ml) was added to two blank controls of either glycinin or β-congly...

The Chryseobacterium sp. kr6 strain has been described as a highly keratinolytic bacterium showing effective feather-degrading and de-hairing activities. A keratinase Q1 enzyme was purified from Chryseobacterium sp. kr6 culture by Phenyl Sepharose and Superose 12HR chromatography. This enzyme showed a specific activity of 967U/mg for keratin azure. Electrophoresis under denaturing conditions sh...

The present study was aimed at isolating a potential keratinase producing bacteria from chicken feathers collected from poultry waste sites in and around Coimbatore, Tamil Nadu, and optimising the parameters for the enzyme production. The strain was identified as Bacillus licheniformis KMBVP based on 16srRNA gene sequencing. The strain showed high keratinolytic activity when cultured in feather...

A fungal isolate with capability to grow in keratinous substrate as only source of carbon and nitrogen was identified as Aspergillus niger using the sequencing of the ITS region of the rDNA. This strain produced a slightly acid keratinase and an acid protease during cultivation in feather meal. The peak of keratinolytic activity occurred in 48 h and the maximum proteolytic activity in 96 h. The...

Two hundred thirty four fungal strains were isolated by baiting method, for their feather degradation and keratinase producing ability. Fungi were tested on solid milk agar plates and in submerged culture. Maximum clearing zone was made by Chrysosporium indicum (7mm) on solid agar plates. The highest keratinase production was found in case of Acremonium strictum (74.40Unit/mL & 124.72 Unit/ml i...