1 2 Amino acids are polymerized into peptides in the peptidyl transferase center of the 3 ribosome. The nascent peptides then pass through the exit tunnel before they reach the 4 extra-ribosomal environment. A number of nascent peptides interact with the exit tunnel 5 and stall elongation at specific sites within their peptide chain. Several mutational 6 changes in RNA and protein components of...

Article history: Received 23 October 2010 Received in revised form 4 January 2012 Accepted 6 January 2012 Available online 14 January 2012 JEL classification: F10 F12 F14 F16 L22 J24

The mechanism of resistance to the streptogramin antibiotics quinupristin and dalfopristin was studied in a Staphylococcus aureus clinical isolate selected under quinupristin-dalfopristin therapy, in four derivatives of S. aureus RN4220 selected in vitro, and in a mutant selected in a model of rabbit aortic endocarditis. For all strains the MICs of erythromycin, quinupristin, and quinupristin-d...

Crystal structures of H. marismortui large ribosomal subunits containing the mutation G2099A (A2058 in E. coli) with erythromycin, azithromycin, clindamycin, virginiamycin S, and telithromycin bound explain why eubacterial ribosomes containing the mutation A2058G are resistant to them. Azithromycin binds almost identically to both G2099A and wild-type subunits, but the erythromycin affinity inc...

The spinach chloroplast ribosomal protein (r-protein) CL22 contains a central region homologous to the Escherichia coli r-protein L22 plus long N- and C-terminal extensions. We show in this study that the CL22 combines two properties which in E. coli ribosome are split between two separate proteins. The CL22 which binds to the 5S rRNA can also be linked to an erythromycin derivative added to th...

The proteins of the large subunit of rat liver ribosomes were separated into seven groups by stepwise elution from carboxymethylcellulose with LiCl at pH 6.5. Seventeen proteins (L4, L5, Li’, L9, Lll, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35’, L37, and L39) were isolated from three of the groups (B60, D60, G60) by ion exchange chromatography on carboxymethylcellulose and by filtration t...

The proteins of the large subunit of rat liver ribosomes were separated into seven groups by stepwise elution from carboxymethylcellulose with LiCl at pH 6.5. Seventeen proteins (L4, L5, Li’, L9, Lll, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35’, L37, and L39) were isolated from three of the groups (B60, D60, G60) by ion exchange chromatography on carboxymethylcellulose and by filtration t...

Guruswami and Sinop [11] give a O(1/δ) approximation guarantee for the non-uniform Sparsest Cut problem by solving O(r)-level Lasserre semidefinite constraints, provided that the generalized eigenvalues of the Laplacians of the cost and demand graphs satisfy a certain spectral condition, namely, λr+1 ≥ Φ∗/(1 − δ). Their key idea is a rounding technique that first maps a vector-valued solution t...