INTRODUCTION During anaphase B in mitosis, polymerization and sliding of overlapping spindle microtubules (MTs) contribute to the outward movement the spindle pole bodies (SPBs). To probe the mechanism of spindle elongation, we combine fluorescence microscopy, photobleaching, and laser microsurgery in the fission yeast Schizosaccharomyces pombe. RESULTS We demonstrate that a green laser cuts ...

This chapter reviews the optics of pulsed laser microbeams and the use of basic instrumentation to provide pulsed laser microbeam capabilities within a microscope platform. Moreover, we review the principal mechanisms by which laser microbeams produce microsurgical effects in cellular targets. We discuss the principal photothermal, photomechanical, and photochemical damage mechanisms as well as...

Background: Vestibular Schwannoma (VS) is one of the skull base tumors originating from vestibular portion of eighth cranial nerve. Recently, 2 micro-Thulium laser is used in the surgery of some intracranial tumors. Objectives: Assessing the efficacy of 2 micro-Thulium flexible hand-held laser fiber (RevolixjrÒ) in microsurgical removal of VS. Materials and Methods: This retrospect...

We demonstrate an endoscope-compatible single-fiber-based device that performs simultaneous confocal microscopy and high-precision laser microsurgery. The method is based on mapping of two-dimensional sample coordinates onto the optical spectrum and allows us to perform two-dimensional imaging and microsurgery without any mechanical movement of the probe or the sample. The technology holds prom...

The increased application of the da Vinci robotic platform (Intuitive Surgical Inc.) for microsurgery has led to the development of new adjunctive surgical instrumentation. In microsurgery, the robotic platform can provide high definition 12×-15× digital magnification, broader range of motion, fine instrument handling with decreased tremor, reduced surgeon fatigue, and improved surgical product...

BACKGROUND Early glottic cancer can be cured with transoral laser resection, but in cases with anterior commissure involvement, there is still controversy concerning the best treatment modality. METHODS The impact of anterior commissure involvement on local control was analyzed in a retrospective review of 444 patients with early glottic cancer (pT1a-pT2a) treated between 1986 and 2004 with t...

Laser killing of cell nuclei has long been a powerful means of examining the roles of individual cells in C. elegans. Advances in genetics, laser technology, and imaging have further expanded the capabilities and usefulness of laser surgery. Here, we review the implementation and application of currently used methods for target edoptical disruption in C. elegans.