نتایج جستجو برای: leishmaniasis species identification
تعداد نتایج: 934797 فیلتر نتایج به سال:
Human visceral leishmaniasis (VL) caused by L. infantum and cutaneous leishmaniasis (CL) caused by L. tropica and L. infantum have been reported in Turkey. L. infantum is also responsible for canine leishmaniasis (CanL) and it is widely common in the country. The main aim of the present study was to design a real-time PCR method based on the internal transcribed spacer 1 (ITS1) region in the di...
Background and Objective: Leishmaniasis is one of the most important vector-borne zoonosis that transmits to human by sand fly species. The current study was carried out to identify sand fly species in Zirkooh, Khorasan-e-Jonobi Province. It was also aimed to determine Leishmania species in infected sand flies by RFLP-PCR method. Materials and methods:Sand fly specimens were collected by usi...
during 2010-2011 a faunistic survey was carried out for collection and identification of edaphic mesostigmatic mites (acari: mesostigmata) in jiroft and suburb. mites were extracted from soil samples by berlese funnel. the mites were cleared in nesbit fluid and were mounted in hoyer medium on microscopic slides. in this study 43 spices from 26 genera and 16 families were collected and identifie...
during our study (summer of 1994) twenty two tatera indica were collected and two out of them (9.09%) were naturally infected with amastigote. parasites were cultured in nnn+lit medium and isolated promastigotes were sent to the medical university of montpellier in france for identification. the result of isoenzyme showed, the parasite was leishmania major zymodem mon 26-(=lon 1), therefore tat...
The discrimination of Leishmania species from patient samples has epidemiological and clinical relevance. In this study, different gene target PCR-restriction fragment length polymorphism (RFLP) protocols were evaluated for their robustness as Leishmania species discriminators in 61 patients with cutaneous leishmaniasis. We modified the hsp70-PCR-RFLP protocol and found it to be the most reliab...
BACKGROUND Leishmaniasis is a zoonotic disease caused by species of protozoa of the genus Leishmania. In recent years, incidence of cutaneous leishmaniasis has increasing trend in Golestan Province, North of Iran. The aim of the present study was to identify the frequency of cutaneous leishmaniasis using PCR-RFLP in patients referred to Kalaleh Health Center, during 2013-14. METHODS This desc...
We surveyed areas of the state of Rondônia in western Amazon for phlebotomine, which are potential vectors of leishmaniasis. A total of 5,998 specimens were captured, resulting in the identification of 48 species within the Lutzomyia (99.98%) and Brumptomyia (0.02%) genera. The predominant species was Lutzomyia davisi, followed by Lutzomyia umbratilis, Lutzomyia llanosmartinsi, Lutzomyia c. car...
Introduction: Annually, more than 14 million people are reported to be infected with Leishmaniasis all over the world. In Iran, this disease is seen in the form of cutaneous and visceral leishmaniasis, of which the cutaneous form is more wide spread. In recent years, cutaneous leishmaniaisis is diagnosed by PCR utilizing specific primers in order to amplify different parasite genes including ri...
background: the aim of this study was to assess leishmania infection in sand fly species from areas where leishmaniasis is endemic. this is important for prediction of the risk and expansion of the disease. methods: in this cross-sectional study we used a pcr-based method for detection of leishmania minicircle dna within individual sand flies from orzoieh, a new endemic leishmaniasis focus in s...
BACKGROUND & OBJECTIVES Rapid and accurate diagnosis and identification of Leishmania sp causing cutaneous leishmaniasis is crucial in control and therapeutic programs. The problem of diagnosis with traditional methods is that they have a low sensitivity or time consuming but molecular techniques would be an alternative method for rapid and accurate diagnosis. In this work, tryparedoxine peroxi...
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