Prediction using pure standards is expected to be biased whenever the slope of the calibration is affected by the presence of sample matrix. Moreover, in the presence of unknown spectral interferents, first-order algorithms like partial least squares cannot be used. In this study, a method for determination of carvedilol (CAR) in tablet and urine samples is proposed by excitation-emission fluor...

The interaction of a new derivative of thiazole orange (TO-3) with calf thymus DNA (ctDNA) has been investigated by fluorescence and absorption spectroscopy. When TO-3 binds to ctDNA, absorption bands exhibit significant hypochromicity at low base pair/dye ratio (BP/D ratio), and high BP/D show hyperchromicity with red shift. The spectral changes are attributed to the different species formed b...

To date no direct methods are available for the subunit quantification of pheomelanin, sulfur-containing melanin form whose structure increases melanoma risk. Here we apply Raman spectroscopy combined with chemometrics (MCR-ALS method) to quantify benzothiazine (BT) and benzothiazole (BZ), constituent monomers pheomelanin. The spectra synthetic pheomelanins only composed BT or BZ were markedly ...

For enzyme-catalysed biotransformations, continuous in situ detection methods minimise the need for sample manipulation, ultimately leading to more accurate real-time kinetic determinations of substrate(s) and product(s). We have established for the first time an on-line, real-time quantitative approach to monitor simultaneously multiple biotransformations based on UV resonance Raman (UVRR) spe...

Pasta is mostly composed by wheat flour and water. Nevertheless, can be partially replaced fibers to provide extra nutrients in the diet. However, fiber affect technological quality of pasta if not properly distributed. Usually, determinations parameters are destructive time-consuming. The use Near Infrared-Hyperspectral Imaging (NIR-HSI), together with machine learning methods, valuable improv...

a new method was developed for the spectral resolution by further determination of three- and  four-component  mixtures  of  drugs in urine samples through  the complementary application of multivariate curve resolution-alternating least squares with correlation  constraint. in the current study, a simple method was proposed to construct a calibration set for the mixture of drugs in the presenc...

Three-dimensional reconstruction in brightfield microscopy is challenging since a 2D image includes from in-focus and out-of-focus light which removes the details of specimen’s structures. To overcome this problem, many techniques exist, but these generally require an appropriate model Point Spread Function (PSF). Here, we propose new images restoration method based on application Multivariate ...