BACKGROUND Human myeloid dendritic cells (MDC) have been identified in human solid tumor tissue of head and neck squamous cell carcinoma (HNSCC), their cellular functions being strongly affected in this environment. The characterization of MDC differentiation and functions requires the establishment of highly efficient isolation procedures. MATERIALS AND METHODS Human MDC were isolated from p...

The effects of gamma-irradiation on the differentiation of peripheral blood monocytes (PBM) into monocyte derived dendritic cells (MDC), their maturation, and subsequent ability to present antigen to T cells was studied. Undifferentiated MDC were more sensitive to gamma-irradiation induced apoptosis than mature MDC. Irradiation of immature MDC with 5 Gy of gamma-rays down regulated the expressi...

BACKGROUND During early HIV-1 infection (EHI), the interaction between the immune response and the virus determines disease progression. Although CD1c + myeloid dendritic cells (mDCs) can trigger the immune response, the relationship between CD1c + mDC alteration and disease progression has not yet been defined. METHODS EHI changes in CD1c + mDC counts, surface marker (CD40, CD86, CD83) expre...

Effective therapeutic vaccines contain two primary constituents, antigen and adjuvant. Adjuvants consisting of microbial pattern molecules play a central role in vaccination. Successful vaccine requires efficient induction of antibody (Ab), type I interferons (IFN), cytokines/chemokines, cytotoxic T lymphocytes (CTL) and/or NK cells. Toll-like receptors (TLRs) in myeloid dendritic cells (mDC) e...

The effects of adding mechanically deboned chicken (MDC) hydrolysates on the quality characteristics of imitation crab stick (ICS) during storage were investigated. ICS was prepared from Alaska Pollack, chicken breast surimi, and protein hydrolysates enzymatically extracted from MDC. ICS samples were divided into 4 groups: without protein hydrolysate (control), added with 0.5% protein hydrolysa...

Membrane distillation crystallization (MDC) is an emerging technology envisaged to manage challenges affecting the desalination industry. This can sustainably treat concentrated solutions of produced water and industrially discharged saline wastewater. Simultaneous recovery clean minerals achieved through integration membrane (MD). MDC has received vast research interest because its potential h...

The human immunodeficiency virus type–1 (HIV-1) uses the chemokine receptors CCR5 or CXCR4 (in conjunction with CD4) to enter healthy cells, and the chemokine ligands to these receptors generally block virus infection (1–3). R. Pal et al. studied a truncated form of the b-chemokine macrophage-derived chemokine (MDC), which lacked the first two NH2-terminal amino acids [(22)MDC] that they purifi...

Gene expression in murine dendritic cells (DCs) infected with green fluorescent protein-expressing Salmonella enterica serovar Typhimurium BRD509 was studied by mRNA differential display. Infected DCs were sorted from uninfected cells by flow cytometry. The mRNA expression patterns of infected and uninfected cells revealed a number of differentially expressed transcripts, which included the mac...

BACKGROUND Histamine is an important mediator of allergic diseases. It modulates the cytokine expression of various subtypes of antigen-presenting cells by four known receptors, H1R-H4R. The effects of histamine on myeloid dendritic cells (mDC) are unclear. METHODS Monocytes and mDC were isolated from human PBMC. Histamine receptor expression was evaluated by real-time PCR. Cells were stimula...