introduction: human embryonic stem cells (hescs) are pluripotent cells that can proliferate and differentiate to many cell types. their electrophysiological properties have not yet been chracterzed. in this study, the passive properties (such as resting membrane potential, input resistance and capacitance) and the contribution of delayed rectifier k+ channel currents to the membrane conductance...

the effect of triton x-100, na cholate and tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of salmonella typhimurium was studied. the detergents were used in various concentrations (1.6 to 64 mm) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. results showed that no inne...

BACKGROUND Mesenchymal stem cells (MSCs) are ideal candidates for treatment of diseases. Amniotic membranes are an inexpensive source of MSCs (AM-MSC) without any donor site morbidity in cell therapy. Adipose tissue derived stem cells (ASCs) are also suitable cells for cell therapy. There is discrepancy in CD271 expression among MSCs from different sources. In this study, the characteristics...

The effect of Triton X-100, Na cholate and Tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of Salmonella typhimurium was studied. The detergents were used in various concentrations (1.6 to 64 mM) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. Results showed that no inne...

Introduction: Human embryonic stem cells (hESCs) are pluripotent cells that can proliferate and differentiate to many cell types. Their electrophysiological properties have not yet been chracterzed. In this study, the passive properties (such as resting membrane potential, input resistance and capacitance) and the contribution of delayed rectifier K+ channel currents to the membrane conducta...

background: mechanotransduction plays a pivotal role in remodeling and repair of skeletal tissues. this mechanism has been widely used in bone tissue engineering especially under in vitro conditions. to date, various stem cells have been used for this purpose. the present study was thefirst to evaluate the effect of mechanical loading on differentiation of human endometrial stemcells (hescs) to...

application of nanotechnology in medicinal and biological fields has attracted a great interest in the recent yeras. in this paper the cell membrane leakage induced by iron nanoparticles (fe-np) against pc12 cell line which is known as a model of nervous system cell line was investigated by the lactate dehydrogenase (ldh) test. therefore, pc12 cells were incubated with different concentration o...

objective: envenomation by heamotoxic snakes constituted a critical health occurrence in the world. bleeding is the most sever consequence following snake bite with viperid and crothalid snakes. it is believed that the degradation of vascular membrane caused hemorrhage; in contrast, some suggested that direct cytotoxicity has role in endothelial cell disturbances. this study was to evaluate the...