Methods This prospective study was done at NMC, Nellore from July 2008-December 2009. Samples received at microbiology lab for acid fast staining were included in this study. Smears were stained by Ziehl-Neelsen’s technique. Samples were cultured on Lowenstein-Jensen media after processing by modified Petroff’s method and incubated according to CLSI guidelines. Identification of Mycobaterium tu...

A case of nocardiosis in a post-renal transplant patient is being reported for the first time from Eastern India. The patient had multiple abscesses on the right thigh and chest. Direct examination of the aspirated pus by Gram stain and modified Ziehl Neelsen stain revealed gram-positive beaded, partially acid-fast, branching filaments. Culture of the pus yielded Nocardia asteroides. The patien...

The prevalence of Cryptosporidium infection among lambs and goat kids in Serbia was examined. The animals were grouped according to age, i.e., up to 30 days and from 31 to 90 days. The infection was diagnosed using three coprological procedures. Sheather’s sugar flotation was employed to determine the concentration of oocysts. Modified Ziehl-Neelsen technique and modified Kinyoun technique were...

To investigate the prevalence of Cryptosporidium infection in horses, a total of 200 fecal specimens were collected randomly from five farms in Tehran. Samples were taken during a year, 50 specimens each season. Of these specimens, 57 were obtained from Caspian Pony. The presence of oocysts in the samples was confirmed by modified Ziehl-Neelsen staining of direct smears of the fecal material fr...

A total of 320 faecal specimens obtained from 295 patients (192 children and 103 adults) with diarrhoea and 47 faecal specimens from healthy adults were screened for Cryptosporidium oocysts. Faecal specimens were first screened by modified Ziehl Neelsen stain and specimens with oocysts were confirmed by direct immunofluorescence technique. Cryptosporidium oocysts were detected in 4 children but...

We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a speci...

Methods: Blood, urine, and stool samples were collected from 687 hospitalized children with diarrhoea in Basrah, Iraq. The causative agents (Bacteria and parasites) for diarrhoea were identified, especially E.coli O157: H7 which was subjected to serotyping by fluorescent technique. Direct smear; Ritchie formalin– ether–sedimentation concentration and modified Ziehl-Neelsen staining techniques w...

In developing countries pulmonary tuberculosis is usually diagnosed by detecting acid-fast bacilli (AFB) in sputum using a Ziehl-Neelsen (Z-N) staining method. However, in the field the traditional method of staining is difficult to carry out. This study evaluates the efficiency of two cold staining methods, namely Gabbet’s and a modified two reagent cold staining method compared with the Z-N t...

The stool samples from 363 diarrhoeic and non- diarrhoeic children and adults from several locations in two towns of west Azerbaijan area of Iran examined for Cryptosporidium infection by modified Ziehl- Neelsen technique revealed the presence of infection in 7.66% of diarrhoeic patients and in none of the non- diarrhoeic individuals. The percentage of detection was higher among females ( ...