An antifungal bacterial strain, isolated from a greenhouse soil sample, inhibits growth of microflora nearby. It was selected for further studies of bacterial antifungal properties. This isolate was identified as a Pseudomonas sp. based on carbohydrate utilization, and other biochemical and physiological tests. Petri plate assay revealed that the Pseudomonas sp. exhibited antifungal activity ag...

Root rot is an important and widespread disease that often causes significant reduction in the yield and quality of harvested peas throughout the production areas in New York. Pea root rot can be caused by one of several soilborne pathogens or various combinations of root pathogens and also non-pathogens. In recent years, Fusarium solani f. sp. pisi (causal pathogen of Fusarium root rot) has be...

From the seeds of Acacia confusa, a chitinase-like antifungal protein designated as acaconin that demonstrated antifungal activity toward Rhizoctonia solani with an IC₅₀ of 30±4 µM was isolated. Acaconin demonstrated an N-terminal sequence with pronounced similarity to chitinases and a molecular mass of 32 kDa. It was isolated by chromatography on Q-Sepharose, SP-Sepharose and Superdex 75 and w...

The antifungal effect of 10 plant extracts viz., Thevetia peruviana, Ocimum basilicum, Piper betel, Murraya koenigii, Chrysanthemum coronarium, Polyalthia longifolia, Catharanthus roseus, Pelargonium graveolens, Moringa officinalis and Lawsonia inermis were evaluated by poisoned food technique against Rhizoctonia solani Kuhn, the causal organism of sore shin disease of tobacco. Among all the pl...

Rhizoctonia solani and R. oryzae are the principal causal agents of Rhizoctonia root rot in dryland cereal production systems of the Pacific Northwest. To facilitate the identification and quantification of these pathogens in agricultural samples, we developed SYBR Green I-based real-time quantitative-polymerase chain reaction (Q-PCR) assays specific to internal transcribed spacers ITS1 and ITS...

This study investigated lytic enzyme activities in three indigenous Trichoderma strains namely, Trichoderma asperellum, Trichoderma harzianum and Trichoderma sp. Native Trichoderma strains and a virulent strain of Rhizoctonia solani isolated from infected bean plants were also included in the study. Enzyme activities were determined by measuring sugar reduction by dinitrosalicylic acid (DNS) me...