نتایج جستجو برای: optimized expression
تعداد نتایج: 960581 فیلتر نتایج به سال:
patients and methods in this cross-sectional study, peripheral mononuclear cells (pbmcs) were isolated from peripheral blood of 29 patients with newly diagnosed pulmonary tb and 19 patients with positive tuberculin skin test. the pbmcs were activated with ppd for 72 hours. activated cells were harvested, rna was extracted and cdna was synthesized. a real-time taqman method was designed and opti...
Background and Objective: In recent years, the biological methods have been considered as a most promising candidate for the cancer treatment. One of the most applicable methods is recombinant proteins. ONTAK immunotoxin is a special drug for cancer therapy of leukemia, lymphoma and melanoma. With respect to the dramatic growth of cancer in today's society, production of recombinant prot...
background: influenza virus is the major cause of lower respiratory tract illnesses on the worldwide. vaccination can be an effective tool to prevent its outbreak. highly conserved viral nucleoprotein is an effective vaccine candidate to provide heterosubtypic immunity, offering resistance against various influenza virus strains. m aterials and methods: in present research np gene was inserted ...
codon optimization, cloning and expression of the human leukemia inhibitory factor (hlif) in e. coli
background: leukemia inhibitor factor (lif) is a very important pleiotropic cytokine which belongs to interleukin-6 (il-6) family. lif exerts multiple effects on different types of cells and tissues with numerous regulatory effects in vivo and in vitro. it is a lymphoid factor, which performs a number of activities including cholinergic neuron differentiation, control of stem cell pluripotency...
Background and Objective: Uricase (EC 1.7.3.3) is an enzyme which catalyses uric acid to H2O2 and alantoin and is utilized in the treatment of hyperuricemia or gout. The aim of this study was cloning of Aspergillus flavus uricase synthetic gene into pET-28(a) and its recombinant expression in E. coli Materials and Methods: The coding sequence of uricase retrieved from gene bank and once opti...
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