the dentate gyrus of hippocampus has long been considered as a focal point for studies on mechanisms responsible for the development of temporal lobe epilepsy (tle). change in intrinsic properties of dentate gyrus granule cells (gcs) has been considered as an important factor responsible in temporal lobe seizures. in this study, we evaluated the intrinsic properties of gcs, during acute phase o...

The present study was conducted to test our hypothesis that the large-conductance calcium-activated potassium channels (BK(Ca) channels) exist in the neurons of the pre-Bötzinger complex (PBC), a brainstem region that may generate respiratory rhythm in mammals, and play roles in central regulation of respiratory activity in neonatal rats. Immunohistochemical technique revealed that BK(Ca) chann...

Penicillium paxilli ATCC 26601 is an asexual filamentous fungal species known for its production of the mycotoxin paxilline. We present here the 35-Mb draft genome sequence for this organism.

[structure: see text] Two novel antiinsectan indole diterpenoids, thiersinines A (1) and B (2), along with seven known paxilline-type compounds, were isolated from organic extracts of a new Penicillium species (P. thiersii NRRL 28147). The structures of 1 and 2 were determined by analysis of 2D NMR data. Thiersinines A and B possess a unique spirocyclic subunit that is unprecedented in previous...

Elevation of intracellular cAMP and activation of protein kinase A (PKA) lead to activation of large conductance voltage- and Ca(2+)-activated K(+) (BK) channels, thus attenuation of detrusor smooth muscle (DSM) contractility. In this study, we investigated the mechanism by which pharmacological inhibition of cAMP-specific phosphodiesterase 4 (PDE4) with rolipram or Ro-20-1724 (C(15)H(22)N(2)O(...

The pineal glands regulate circadian rhythm through the synthesis and secretion of melatonin. The stimulation of nicotinic acetylcholine receptor due to parasympathetic nerve activity causes an increase in intracellular Ca(2+) concentration and eventually downregulates melatonin production. Our previous report shows that rat pinealocytes have spontaneous and nicotine-induced Ca(2+) oscillations...

BACKGROUND Mitochondrial large-conductance Ca2+-sensitive potassium (mBKCa) channels are involved in myocardial ischemic preconditioning. Their role in sildenafil-induced cardioprotection is unknown. We investigated whether sildenafil-induced acute cardioprotection is mediated by activation of mBKCa channels in the rat heart in vitro. METHODS Male Wistar rats (n = 8 per group) were randomized...

Malysz J, Afeli SA, Provence A, Petkov GV. Ethanol-mediated relaxation of guinea pig urinary bladder smooth muscle: involvement of BK and L-type Ca channels. Am J Physiol Cell Physiol 306: C45–C58, 2014. First published October 23, 2013; doi:10.1152/ajpcell.00047.2013.—Mechanisms underlying ethanol (EtOH)-induced detrusor smooth muscle (DSM) relaxation and increased urinary bladder capacity rem...

BACKGROUND Mitochondrial calcium-sensitive potassium (mK(Ca)) channels are involved in cardiac preconditioning. In the present study, we investigated whether also ischaemic-, morphine-induced post-conditioning, or both is mediated by the activation of mK(Ca) channels in the rat heart in vitro. METHODS Animals were treated in compliance with institutional and national guidelines. Male Wistar r...