نتایج جستجو برای: paxilline

تعداد نتایج: 166  

2010
Yu Zhou Qiong-Yao Tang Xiao-Ming Xia Christopher J. Lingle

The tremorogenic fungal metabolite, paxilline, is widely used as a potent and relatively specific blocker of Ca(2+)- and voltage-activated Slo1 (or BK) K(+) channels. The pH-regulated Slo3 K(+) channel, a Slo1 homologue, is resistant to blockade by paxilline. Taking advantage of the marked differences in paxilline sensitivity and the homology between subunits, we have examined the paxilline sen...

2014
Yu Zhou Christopher J. Lingle

Paxilline, a tremorogenic fungal alkaloid, potently inhibits large conductance Ca(2+)- and voltage-activated K(+) (BK)-type channels, but little is known about the mechanism underlying this inhibition. Here we show that inhibition is inversely dependent on BK channel open probability (Po), and is fully relieved by conditions that increase Po, even in the constant presence of paxilline. Manipula...

2013
Barry Scott Carolyn A. Young Sanjay Saikia Lisa K. McMillan Brendon J. Monahan Albert Koulman Jonathan Astin Carla J. Eaton Andrea Bryant Ruth E. Wrenn Sarah C. Finch Brian A. Tapper Emily J. Parker Geoffrey B. Jameson

The indole-diterpene paxilline is an abundant secondary metabolite synthesized by Penicillium paxilli. In total, 21 genes have been identified at the PAX locus of which six have been previously confirmed to have a functional role in paxilline biosynthesis. A combination of bioinformatics, gene expression and targeted gene replacement analyses were used to define the boundaries of the PAX gene c...

The anticonvulsant effect of cannabinoid compound has been shown in various models of seizure. On the other hand, there are controversial findings about the role of large conductance calcium-activated potassium (BK) channels in the pathogenesis of epilepsy. In this study, the effect of arachidonyl-2′-chloroethylamide (ACEA), a CB1 receptor agonist, and a BK channel antagonist, paxilline, either...

The anticonvulsant effect of cannabinoid compound has been shown in various models of seizure. On the other hand, there are controversial findings about the role of large conductance calcium-activated potassium (BK) channels in the pathogenesis of epilepsy. In this study, the effect of arachidonyl-2′-chloroethylamide (ACEA), a CB1 receptor agonist, and a BK channel antagonist, paxilline, either...

2017
Sina Asaadi Mohammad Jahanbakhshi Mahmoud Lotfinia Nima Naderi

The anticonvulsant effect of cannabinoid compound has been shown in various models of seizure. On the other hand, there are controversial findings about the role of large conductance calcium-activated potassium (BK) channels in the pathogenesis of epilepsy. Also, there is no data regarding the effect of co-administration of cannabinoid type 1 (CB1) receptor agonists and BK channels antagonists ...

Journal: :Journal of Biological Chemistry 2007

Journal: :The Journal of physiology 2010
Khaled M Houamed Ian R Sweet Leslie S Satin

BK channels are large unitary conductance K(+) channels cooperatively activated by intracellular calcium and membrane depolarisation. We show that BK channels regulate electrical activity in β-cells of mouse pancreatic islets exposed to elevated glucose. In 11.1 mM glucose, the non-peptidyl BK channel blocker paxilline increased the height of β-cell action potentials (APs) by 21 mV without affe...

Journal: :The Journal of pharmacology and experimental therapeutics 2003
Kelli S Bramlett Keith A Houck Kristen M Borchert Michele S Dowless Palaniappan Kulanthaivel Youyan Zhang Thomas P Beyer Robert Schmidt Jeffrey S Thomas Laura F Michael Robert Barr Chahrzad Montrose Patrick I Eacho Guoqing Cao Thomas P Burris

Natural products have been identified as ligands for a number of members of the nuclear hormone receptor (NHR) superfamily. Often these natural products are used as dietary supplements to treat myriad ailments ranging from perimenopausal hot flashes to hypercholesterolemia and reduced cognitive function. Examples of some natural product ligands for NHRs include genestein (estrogen receptors NR3...

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