نتایج جستجو برای: pcr semi
تعداد نتایج: 314764 فیلتر نتایج به سال:
بیماری تیلریوز یکی از مشکلات مهم در عدم پیشرفت صنعت دامپروری در مناطق گرمسیری و نیمه گرمسیری جهان است.تیلریوز یکی از بیماری های مهم تک یاخته ای خونی منتقله از راه کنه می باشد که در گاو، گوسفند، بز و سایر نشخوارکنندگان وحشی گزارش شده است. تیلریا لستوکاردی و تیلریا اویس به عنوان عوامل ایجاد کننده تیلریوز گوسفندی در ایران شناخته شده اند اما به لحاظ اپیدمیولوژیک وضعیت این بیماری در ایران به خوبی شن...
objective: inhibition of apoptosis may favor the onset and progression of cancer. survivin is an inhibitor of apoptosis that has been considered as a potential marker for diagnostic and/or prognostic of bladder cancer. the survivin protein regulates both cell division and cell death and is overexpressed in the vast majority of human cancers. in this study, the expression pattern and potential p...
INTRODUCTION Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. METHODS We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. RESULTS The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or ...
objective: gbv-c is a virus transmissible via blood transfusion and sexual routes. because of the shared transmission routes in gbv-c and hepatitis b virus (hbv) we have attempted to detect gbv-c in hbsag-positive patients using the more sensitive semi-nested pcr. we used restriction fragment length polymorphism (rflp) to determine genotype. methods: a total of 100 serum samples were collected...
babesiosis is an important tickborne disease in the sheep of iran. objectives: a molecular study was carried out in north khorasan province, iran in 2010-2011, designed to identify babesia spp. infection of both sheep and ticks. methods: ninety sheep from different flocks were clinically examined and blood samples were collected with ixodid ticks . the collected ticks were separated into 82 ti...
a molecular survey was performed for identification theileria spp in sheep and ticks during from 2010-2011 in fasa and kazeroun areas, fars province, iran. a total of 100 sheep from different flocks were clinically examined and blood samples with ixodid ticks collected. the prepared blood smears from capillary vein of ear were stained with giemsa methods and examined by using light microscope. ...
background and objectives: the aim of this study was to compare direct microscopic examination with culture and pcr for the diagnosis of mucorales infection in blood and tissue specimens. material and methods: blood samples and tissue specimens were obtained from 28 patients (total 58 samples) with suspected invasive fungal infection and cultured on proper media. direct smear of tissue samples ...
introduction: brain-derived neurotrophic factor (bdnf) and its receptor, trkb, in the hippocampus are targets for adverse effects of stress paradigms in addition, bdnf and its receptor play key role in the pathology of brain diseases like depression. in the present study, we evaluated the possible role of hippocampal bdnf in depression during pregnancy, methods: to achieve the purpose, repeated...
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