Amaç: Bu çalışma, domates genetik kaynaklarının kuraklık stresine karşı hızlı taranması için PEG-6000'in neden olduğu stresinin uygunluğunu belirlemek amacıyla yapılmıştır. Materyal ve Yöntem: Çalışmada, kurağa toleransı yüksek (M28) düşük (Alyans) bilinen iki çeşit test edilmiştir. Çimlendirme testinde farklı PEG konsantrasyonu (%4 %6) denenmiştir. Fide aşamasındaki su kültürü tekniği ile gerç...

Four methods of extraction and three methods of concentration of three enteric viruses from mussels were comparatively evaluated by reverse transcriptase PCR (RT-PCR). Shellfish were experimentally contaminated by immersion in seawater seeded with astrovirus, hepatitis A virus, or poliovirus. Sixty-gram samples of mussel tissues were processed by using borate buffer, glycine solution, saline be...

growing applications of phenylalanine dehydrogenase (phedh) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. with goal of finding a new technique, the extraction and purification of recombinant bacillus badius phedh in polyethylene glycol 6000 (peg-6000) and ammonium sulfate aqueous two-phase systems...

Calcification is a frequent cause of the clinical failure of bioprosthetic heart valves fabricated from glutaraldehyde pretreated bovine pericardium (GATBP). An investigation was made of the grafting of different molecular weight polyethylene glycol (PEG 600, 1500, 4000 and 6000) via glutaraldehyde (GA) linkages to bovine pericardium (BP) and of their stability and calcification. The process of...

hbs vaccines were prepared by three different methods: 1) heat inactivation (krugman, et.al 1971)2) ammonium sulphate precipitation followed by column chromatography (sephadex g-200) 3) peg-6000 (polyethylene glycol) precipitation followed by column chromatography using sephadex g-200. their efficacy was studied in guinea pigs, and the results compared with commercial japanese vaccine (green cr...

Seed germination is generally the critical step in seed establishment and thus determination of successful crop production. This study was focused at examination biochemical parameters effected by low water potential which generated polyethylene glycol (PEG) 6000 mannitol, related to drought stress growth Waha durum wheat genotype. Two tests were carried out a chamber; first comprises into Petr...

Growing applications of phenylalanine dehydrogenase (PheDH) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. With goal of finding a new technique, the extraction and purification of recombinant Bacillus badius PheDH in polyethylene glycol 6000 (PEG-6000) and ammonium sulfate aqueous two-phase systems...

Solid Dispersions were prepared by solvent evaporation technique using microcrystalline cellulose, PEG 4000, PEG 6000, PVP-K30 and Gelucire 50/13. Further objective of the project was to develop fast release surface solid dispersion of glibenclamide and compressed at low pressure to form fast-melting pharmaceutical tablet. In this study, we used sodium starch glycolate for mouth dissolving natu...

Polyethylene glycol (PEG) can be used to mimic osmotic stress in plant tissue cultures to study mechanisms of tolerance. The aim of this experiment was to investigate the effects of PEG (M.W. 6000) on embryogenic callus of Medicago truncatula. Leaf explants were cultured on MS medium with 2 mg L-1 NAA and 0.5 mg L-1 BAP for 5 months. Then, calli were transferred to the same medium further suppl...

Drought is one of the most detrimental environmental stressors to plants with potential decrease crop yields and affect agricultural sustainability. Native bacteria beneficial traits enhance plant growth help avoid reverse effects drought in a greater extent. In present study, we aimed ( i ) isolate drought-tolerant Bacillus isolates from rhizosphere soil wheat grown at different locations Jais...