Abstract Background The lack of effective treatment against the highly infectious SARS-CoV-2 has aggravated already catastrophic global health issue. Here, in an attempt to design efficient vaccine, a thorough immunoinformatics approach was followed predict most suitable viral proteins epitopes for building that vaccine . Methods amino acid sequences four structural (S, M, N, E) along with one ...

BACKGROUND Lunasin is a chemopreventive peptide produced in a number of plant species. It comprises a helical region with homology to a region of chromatin binding proteins, an Arg-Gly-Asp cell adhesion motif and eight aspartic acid residues. In vitro studies indicate that lunasin suppresses chemical and oncogene driven transformation of mammalian cells. We have explored efficient recombinant p...

Although the ability of Bifidobacterium spp. to grow on fructose as a unique carbon source has been demonstrated, the enzyme(s) needed to incorporate fructose into a catabolic pathway has hitherto not been defined. This work demonstrates that intracellular fructose is metabolized via the fructose-6-P phosphoketolase pathway and suggests that a fructokinase (Frk; EC 2.7.1.4) is the enzyme that i...

In this study, a method for expressing Cryptosporidium hominis GP60 glycoprotein in Escherichia coli for production of polyclonal anti-GP60 IgY in chickens was developed aiming future studies concerning the diagnosis, prevention and treatment of cryptosporidiosis. The full-length nucleotide sequence of the C. hominis gp60 gene was codon-optimized for expression in E. coli and was synthesized in...

Foot-and –Mouth disease virus (FMDV) is a positive sense RNA virus and the genome codes for single polyprotein. The FMDV L protein is located at the N terminus of the polyprotein and is the first gene product released from the nascent polyprotein. The leader L protease which is involved in pathogenesis has two known functions: (i) auto-catalytic removal from the N terminus of the viral polyprot...

introduction: omp31 is animmunodominant and protective antigen conserved in brucella species and a good candidate for vaccine design. material & methods: the present study aimed at in silico design of the truncated omp31 (tomp31) using bioinformatic tools and to express the selected form in escherichia coli (e. coli) results and conclusion: various bioinformatically calculated scores for the mo...

background: angiogenesis is an important process in various physiologic and pathologic states. the most significant stimulator of angiogenesis is vascular endothelial growth factor (vegf). in contrast, vasohibin1 acts as an angiogenesis inhibitor which specifically inhibits new vessels formation. the aim of the present study was cloning and expression of vasohibin1 gene in e. coli as well as pu...

Creatinine amidohydrolase(EC 3.5.2.10) catalyzes the reversible conversion of creatinine to creatine. Creatininase in combination with other enzymes is used for detection of creatinine in serum and urine which is of significant value for detection of renal, muscular and thyroid functions. The aim of this study was to produce recombinant creatininase enzyme in E.coli expression system to use it ...

  Introduction: Omp31 is animmunodominant and protective antigen conserved in Brucella species and a good candidate for vaccine design. Material & methods: The present study aimed at in silico design of the truncated Omp31 (TOmp31) using bioinformatic tools and to express the selected form in Escherichia coli (E. coli) Results and conclusion: Various bioinformatically calculated scores for the ...