Numerous examples of phage display applied to soluble proteins demonstrate the power of the technique for protein engineering, affinity reagent discovery and structure-function studies. Recent reports have expanded phage display to include membrane proteins (MPs). The scope and limitations of MP display remain undefined. Therefore, we report data from the phage display of representative types o...

Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the need to add helper phage by using 'bacterial packaging cell lines' that provi...

In this article are reviewed the promising uses of phage display in areas such as microbial pathogens detection and vaccination. Phage is a molecular technique by which foreign proteins expressed at surface particles. Such phages thereby become vehicles for expression that not only carry within them nucleotide sequence encoding proteins, but have also capability to replicate. Recent data acquir...

M13 bacteriophage display presents polypeptides as fusions to phage coat proteins. Such phage-displayed ligands offer useful reagents for biosensors. Here, we report a modified phage propagation protocol for the consistent and robust display of two different genetically encoded ligands on the major coat protein, P8. The results demonstrate that the phage surface reaches a saturation point for m...

1 Center of Bioinformatics (COBI), Key Laboratory for NeuroInformation of Ministry of Education, University of Electronic Science and Technology of China, Chengdu 610054, China 2Department of Chemistry and Alberta Glycomics Centre, University of Alberta, Edmonton, AB, Canada T6G 2G2 3National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun...

There is an ever-increasing demand to select specific, high-affinity binding molecules against targets of biomedical interest. The success of such selections depends strongly on the design and functional diversity of the library of binding molecules employed, and on the performance of the selection strategy. We recently developed SRP phage display that employs the cotranslational signal recogni...