One of the barriers during whey filtration using UF membrane is the fouling phenomenon of the membrane, which is caused by whey proteins. In this work, the UF membranes were prepared using polysufone (PSf), dimethyl formamide (DMF), 1 wt.% poly vinyl pyrrolidone (PVP) and different concentrations of LiCl via phase inversion induced by immersion precipitation. The prepared membranes were charact...

Molecular interactions of 4-diphenylmethoxy-1-methylpiperidine derivatives with the calcium channel CaV1.1 (pdb:6JP5) are described. All compounds tested, previously shown to inhibit adrenergic vascular contractions, display similar binding energetics and trans-membrane domain 6JP5 on opposite side relative pore, where nifedipine, a known dihydropyridine Ca2+ blocker binds. Additionally, tested...

Nanoporous redox cycling devices are highly efficient tools for the electrochemical sensing of redox-active molecules. By using a redox-active mediator, this concept can be exploited for the detection of molecular binding events via blocking of the redox cycling current within the nanopores. Here, we investigate the influence of different blocking scenarios inside a nanopore on the resulting re...

Hypokalemic periodic paralysis and normokalemic periodic paralysis are caused by mutations of the gating charge-carrying arginine residues in skeletal muscle Na(V)1.4 channels, which induce gating pore current through the mutant voltage sensor domains. Inward sodium currents through the gating pore of mutant R666G are only approximately 1% of central pore current, but substitution of guanidine ...

Curcumin is a novel drug for lung cancer treatment. However, the mechanism underlying the anti-tumor effect of curcumin remains elusive. Previous evidences indicated that, the methylating transferase DNMT1 is downregulated by curcumin, and the transcription factor 21 (TCF21) is suppressed by DNMT1. We hereby attempt to elucidate the correlation between curcumin treatment and TCF21 expression. E...

We report the results of molecular dynamics simulations of translocation of knotted proteins through pores. The protein is pulled into the pore with a constant force, which in many cases leads to the tightening of the knot. Since the radius of tightened knot is larger than that of the pore opening, the tight knot can block the pore thus preventing further translocation of the chain. Analyzing s...

The influenza virus M2 proton-selective ion channel is known to be essential for acidifying the interior of virions during virus uncoating in the lumen of endosomes. The M2 protein is a homotetramer that contains four 19-residue transmembrane (TM) domains. These TM domains are multifunctional, because they contain the channel pore and also anchor the protein in membranes. The M2 protein is gate...

The open-channel structure of the N-methyl-D-aspartate (NMDA) receptor was investigated to explain apparently conflicting interpretations about ionic interactions within the pore. Patch-clamp techniques were applied to tissue-cultured rat hippocampal neurons from the CA1 region. A wide range of ammonium derivatives was studied to learn about the structure of the pore from permeability and open-...

We report experimental measurements of the salt dependence of ion transport and DNA translocation through solid-state nanopores. The ionic conductance shows a three-order-of-magnitude decrease with decreasing salt concentrations from 1 M to 1 muM, strongly deviating from bulk linear behavior. The data are described by a model that accounts for a salt-dependent surface charge of the pore. Subseq...

one of the barriers during whey filtration using uf membrane is the fouling phenomenon of the membrane, which is caused by whey proteins. in this work, the uf membranes were prepared using polysufone (psf), dimethyl formamide (dmf), 1 wt.% poly vinyl pyrrolidone (pvp) and different concentrations of licl via phase inversion induced by immersion precipitation. the prepared membranes were charact...