introduction: this study was initiated to examine the effect of the ampullary and the isthmic primary cell cultures of human oviduct during long-term co-culture on two-cell mouse embryos. materials and methods: using a mechanical procedure, epithelial cells from ampullary (a) and isthmic (i) regions of the human oviduct were isolated and cultured in ham's f-10 with 10% fetal calf serum (fsc). t...

The conditions for growing preneoplastic mammary epithelial cells in primary cell cultures with serum-free factor-defined medium were established in a series of experiments. The cells, grown in Dulbecco's modified Eagle's medium with 0.6 mM Ca2+, epidermal growth factor, transferrin, insulin, and Na2SeO3, were fibroblast free, exhibited saturation densities similar to those of cells grown in se...

A method is described for subdividing S phase cells in asynchronous cell cultures on the basis of replication band patterns produced in chromosomes by bromodeoxyuridine incorporation. The criteria used for cell classification are objective, requiring the presence or absence of specific bands on particular chromosomes, and therefore lead to subdivisions amenable to quantitative analysis and for ...