introduction: the novel development of the (hba) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. the purpose of this study was to evaluate the correlation between hba score and histone deficiency, protamine content and dna fragmentation. methods: during april- march 2012 patients who were enrolled at shiraz-...

background: flow cytometry (fcm) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. fcm provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. during spermiogenesis histones are replaced by prot...

BACKGROUND Etiology of more than half of Recurrent Spontaneous Abortion. The etiology of more than 50 percent of Recurrent Spontaneous Abortions (RSA) cases has been remained unexplained. It is supposed that RSA may have "paternal effect" due to supply 50% of embryonic genomic content by male gamete. OBJECTIVE The aim of present study was to evaluate the role of sperm apoptosis and protamine ...

Chromomycin A3 binds to DNA and Protamine -deficient spermatozoa look bright yellow,whereas normal spermatozoa appear dull yellow.

Background: Varicocele is considered as a common cause of male infertility and can affect spermatogenesis. In addition, it also predisposed sperm to chromatin damage and morphological alternation. Other factors, such as environmental and genetic conditions also predispose sperm to these conditions. To answer this question, we aimed to evaluation of DNA fragmentation and protamine deficiency thr...

objective: surgery is considered the primary treatment for male infertility from clinical varicocele. one of the main events associated with varicocele is excessive production of reactive oxygen species (ros). n-acetyl-l-cysteine (nac), an antioxidant that scavenges free radicals, is considered a supplement to alleviate glutathione (gsh) depletion during oxidative stress. despite beneficial eff...

OBJECTIVE This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. METHODS Semen analysis and a sperm DNA fragmen...

Background: Although sperm selection according to morphologic and motility characteristic, made to some what the fertility successful, but sperm’s normal morphology cannot demonstrate sperm DNA competence. Since the health of sperm DNA is affecting the results of assisted reproductive technology; the aim of this study is to evaluate semen parameters measured by CASA with sperm chromatin abnorma...