we have investigated the effect of theophylline on the kinetics of the catalytic subunit of protein kinase and related factors in lung tissue. the results show that the point of highest concentration of the c subunit of protein kinase which is active in casein phosphorylation is at 3h of incubation time, but in the presence of 100 ilg/ inl and 10µg/ml theophylline, this is shifted to i.s and 2....

The actin cytoskeleton plays critical roles in numerous cellular events and functions, its spatiotemporal dynamics are maintained regulated by several cofactor proteins. MISP/Caprice is a recently reported actin-bundling protein that also involved the progression of mitosis. In this study, we investigated how actin-regulatory function MISP modulated phosphorylation. A series mutation studies de...

• MPK3 preferentially phosphorylates Serine 114 of ABI4. Phosphorylated ABI4 is required for the complementation abi4 mutants. acts primarily as a repressor. The transcription factor ABA-INSENSITIVE(ABI)4 has diverse roles in regulating plant growth, including inhibiting germination and reserve mobilization response to ABA high salinity, seedling growth sugars, lateral root repressing light-ind...

Abstract Lysosomes are dynamic organelles that transported along microtubules bidirectionally via kinesin and dynein motor proteins. Lysosomal positioning, which is determined by the balance of bidirectional lysosomal movement, changes under various conditions affects functions such as autophagy antigen presentation. A recent study Takemasu et al. (Phosphorylation TMEM55B Erk/MAPK regulates pos...

We have investigated the effect of theophylline on the kinetics of the catalytic subunit of protein kinase and related factors in lung tissue. The results show that the point of highest concentration of the C subunit of protein kinase which is active in casein phosphorylation is at 3h of incubation time, but in the presence of 100 Ilg/ InL and 10µg/mL theophylline, this is shifted to I.S an...

Abstract Background Tristetraprolin (TTP) family proteins contain conserved tandem CCCH zinc-finger binding to AU-rich elements and C-terminal NOT1-binding domain. TTP is phosphorylated extensively in cells, its mRNA destabilization activity regulated by protein phosphorylation. Methods We generated an antibody against phospho-Serine316 located at the site examined phosphorylation LPS-stimulate...

Recognition of the molecular mechanisms of cAMP action against DNA damage-induced apoptosis can be useful to improve the efficacy of DNA damaging therapeutic agents. Considering the critical role of bcl-2-associated death promoter (BAD) and p53 proteins in DNA damage -induced apoptosis, the aim of this study was to assess the effect of cAMP-elevating agents on these proteins in doxorubicin-trea...

objective(s): to understand the role of mitochondrial respiration in cisplatin sensitivity, we have employed wild-type and mitochondrial dna depleted rho0 yeast cells. materials and methods: wild type and rho0 yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against cisplatin by monitoring growth curves, oxygen consumption, ph changes ...