The protocorm-like bodies (PLBs) of Cymbidium hookerianum were used as the explants, effects different hormones and hormone concentrations on induction etiolation stems, subsequent regeneration artificial seeds production conservation using stems studied. results showed that most efficient induced by PLBs was obtained adding 0.1 mg/L NAA 6-BA. cultivated medium containing 2.0/0.1 NAA/6-BA highe...

The endangered epiphytic orchid, Dendrobium chrysotoxum, is known for its ornamental and medicinal uses. However, knowledge of this orchid’s symbiotic seed germination, protocorm anatomy, developmental morphology completely unknown. In study, we investigated the process development D. chrysotoxum during germination using anatomical technologies scanning electron microscopy. There are six stages...

BACKGROUND AND AIMS The endangered leafless ghost orchid, Dendrophylax lindenii, one of the most renowned orchids in the world, is difficult to grow under artificial conditions. Published information on asymbiotic and symbiotic (co-culture with a mycobiont) seed germination, seedling anatomy and developmental morphology of this leafless orchid is completely lacking. This information is critical...

Abstract Background Dendrobium officinale , an endangered Chinese herb, possesses extensive therapeutic effects and contains bioactive ingredients such as major polysaccharides, alkaloids, minimal flavonoids. We first obtained the protocorm-like bodies (PLBs) of this plant through tissue culture in order to determine distribution main secondary metabolites each organelle PLBs. then analyzed cor...

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in...