The performance of a commercially available qualitative PCR test for plasma (AMPLICOR CMV Test; Roche Diagnostics) and a quantitative PCR test for plasma and leukocytes (COBAS AMPLICOR CMV MONITOR Test; Roche Diagnostics) was evaluated with samples from 50 blood or marrow allogeneic transplant recipients who received short courses of sequential ganciclovir therapy (2 weeks intravenously followe...

genetically modified (gm) foods hold the key to ending hunger and malnutrition in africa.   due to the increasing number of gmos cultivation and delay in the approval of biosafety law in nigeria, it has become necessary to screen maize products in order to determine the identity of the consumed daily foodstuffs. in this study, dna extraction from raw and processed maize foods sold commercially ...

Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101DNA copies/μL and found 4.8% (14/290) p...

We assessed the relationship between infection with Mycoplasma genitalium, an emerging sexually transmitted pathogen, and cervical shedding of human immunodeficiency virus (HIV)-1 DNA among 303 HIV-1-positive Kenyan women. HIV-1 shedding was detected by qualitative polymerase chain reaction (PCR) in 154 women (51%); M. genitalium was detected by qualitative PCR in 52 (17%), and organism burden ...

BACKGROUND/AIMS The early diagnosis of acute hepatitis A (AHA) is hindered because serum IgM against hepatitis A virus (HAV) can yield false-negative results during the window period. This study evaluated the diagnostic accuracy of a polymerase chain reaction (PCR) kit for HAV RNA for the diagnosis of AHA. METHODS Samples were collected from 136 patients with acute severe hepatitis at their a...

Assay Manufacturer Method Detection limit or dynamic range (IU/mL) Qualitative HCV RNA dection assays AmplicorTM HCV 2.0 Roche Molecular System Manual RT-PCR 50 Cobas AmplicorTM HCV 2.0 Roche Molecular System Semi-automated RT-PCR 50 VersantTM HCV Siemens Health Care Diagnostics Semi-automated TMA 10 Quantitative HCV RNA detection assays AmplicorTM HCV Monitor Roche Molecular System Manual RT-P...

Nucleic acid amplification can provide sensitive and specific molecular detection and quantification. Since its introduction PCR has been the dominant amplification technology; its widespread use has stimulated improvements in enzymes, instrumentation, and analytical methods. Initial use of PCR was largely qualitative: positive target detection, or inference of target absence above a detection ...

We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a Light Cycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis.