نتایج جستجو برای: recombinant plasmid
تعداد نتایج: 142505 فیلتر نتایج به سال:
Background: Thrombopoietin (TPO) is an important cytokine that has a critical role in regulating hematopoietic stem cells (HSCs) proliferation and megakaryocyte differentiation. Because of scares amount of this protein in human plasma, in many biotechnological centers around the world, recombinant production of this protein has been carried out. This study was aiming to gene cloning and express...
Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...
colonization of nontypeable haemophilus influenzae (nthi) in nasopharynx causes respiratory tract disease. in 80% of clinical isolates, hmw proteins are the major adhesions and induce protective antibodies in the hosts. therefore, it can be used as a vaccine candidate. the aim of this study is designing and cloning of the conserved regions of nthi hmw1 core binding domain.in this study, the sta...
adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. in spite of their usefulness, the construction of adenoviral vectors (adv) is a cumbersome and lengthy process that is not readily amenable to the generat...
lyme borreliosis is a tick-born disease caused by the spirochete borrelia burgdorferi. the aim of this research was molecular typing of different strains of b. burgdorferi and protective efficacy of outer surface protein in animal model. seven strains of b. burgdorferi isolated from skin and csf of lyme disease patients were compared using pcr- rflp as well as dna and amino acid sequences. the ...
Background and purpose: Staphylococcus aureus is an important nosocomial pathogen which causes some diseases such as endocarditis, osteomyelitis, pneumonia, toxic shock syndrome, and food poisoning. The excessive and inappropriate use of antibiotics in the treatment of these diseases causes resistance to many antibiotics. Lysostaphin is an effective agent in the treatment of staphylococcal infe...
Background and Aims: Q59L mutant of L-asparaginase enzyme from Escherichia coli (E. coli) has been introduced with lower side effects. This version of the enzyme might have potential applications in the treatment of leukemia patients. We utilized SHuffle T7 strain of E. coli, to produce the mutant enzyme in the presence of chaperone molecules. Materials and Methods: Q59LAsp gene was cloned in...
Background: Pilot and large-scale production of recombinant proteins requires the presence of stable clones capable of producing large quantities of recombinant proteins. Not only the process of selecting stable clones is time consuming, but also the continuous culturing of clones in large-scale production may cause loss of incoming plasmid and recombinant genes. Thus, considering the advanceme...
several research groups have attempted to make recombinant bacteria capable of efficient desulfurization of oil fraction. the main aim of this study was to design a recombinant strain in order to desulfurize dibenzothiophen (dbt) and its components found in petroleum. in this study the pvlt31 vector harboring dszabc genes was transferred into recombinant e. coli bl21 which contained the dszd ge...
To understand the differences in production and secretion patterns between plasmid-harboring and chromosome-integrated recombinant yeasts, the two recombinant Saccharomyces cerevisiae yeasts, containing the structural glucoamylase STA gene and the SUC2 promoter, were investigated. Both systems were regulated by glucose concentration in the culture broth. First, the glucoamylase activity per gen...
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