Based on the potential benefits to human health, there is interest in developing sustainable nutritional strategies to enhance the concentration of long-chain n-3 fatty acids in ruminant-derived foods. Four Aberdeen Angus steers fitted with rumen and duodenal cannulae were used in a 4 x 4 Latin square experiment with 21 d experimental periods to examine the potential of fish oil (FO) in the die...

Fatty acids such as n-3 and n-6 polyunsaturated fatty acids (PUFA) are critical nutrients, used to improve male reproductive performance through modification of fatty acid profile and maintenance of sperm membrane integrity, especially under cold shock or cryopreservation condition. Also, PUFA provide the precursors for prostaglandin synthesis and can modulate the expression patterns of many ke...

1. alpha-[U-(14)C]Linolenic acid was incubated with the rumen contents of sheep and the metabolic products were characterized by thin-layer chromatography, gas-liquid chromatography and absorption spectroscopy in the ultraviolet and infrared. 2. A tentative scheme for the biohydrogenation route to stearic acid is presented. The main pathway is through diconjugated cis-cis-cis-octadecatrienoic a...

Lokenga Badinga and Charles R. Staples Department of Animal Sciences University of Florida Gainesville, Florida Definition and Sources of Conjugated Linoleic Acid Conjugated linoleic acid (CLA) is a collective term describing a mixture of positional and geometrical isomers of linoleic acid (LA, C18:2) involving a double bond at positions 8 and 10, 9 and 11, 10 and 12 or 11 and 13 (Eulitz et al....

An article we wrote for the 1998 Illinois Dairy Report (Can Milk Fat be Beneficial to Your Health?) provided a brief description of conjugated linoleic acid (CLA), a minor component of milk fat. Briefly, CLA is a fatty acid that is synthesized in the rumen during biohydrogenation of linoleic acid (C18:2), the predominant fatty acid in vegetable oils. The pathway of biohydrogenation is C18:2 ? C...

Increasing the polyunsaturated fatty acid (PUFA) composition of milk is acknowledged to be of benefit to consumer health. Despite the high PUFA content of forages, milk fat contains only about 3% of PUFA and only about 0.5% of n-3 fatty acids. This is mainly due to intensive lipid metabolism in the rumen (lipolysis and biohydrogenation) and during conservation (lipolysis and oxidation) such as ...

Because of the potential benefits to human health, there is interest in increasing 18:3n-3, 20:5n-3, 22:6n-6, and cis-9,trans-11 CLA in ruminant foods. Four Aberdeen Angus steers (406 ± 8.2 kg of BW) fitted with ruminal and duodenal cannulas were used in a 4 × 4 Latin square experiment with 21-d periods to examine the potential of fish oil (FO) and linseed oil (LO) in the diet to increase rumin...