Polan, C. E. (North Carolina State of the University of North Carolina, Raleigh), J. J. McNeill, and S. B. Tove. Biohydrogenation of unsaturated fatty acids by rumen bacteria. J. Bacteriol. 88:1056-1064. 1964.-A simple, rapid, specific assay for the biohydrogenation of unsaturated fatty acids was developed. With this assay, it was shown that washed suspensions of mixed rumen bacteria hydrogenat...

Citation: Dewanckele L, Vlaeminck B, Hernandez-Sanabria E, Ruiz-González A, Debruyne S, Jeyanathan J and Fievez V (2018) Rumen Biohydrogenation and Microbial Community Changes Upon Early Life Supplementation of 22:6n-3 Enriched Microalgae to Goats. Front. Microbiol. 9:573. doi: 10.3389/fmicb.2018.00573 Rumen Biohydrogenation and Microbial Community Changes Upon Early Life Supplementation of 22:...

Optimization of the fatty acid composition of ruminant milk and meat is desirable. Dietary supplementation of algae was previously shown to inhibit rumen biohydrogenation, resulting in an altered milk fatty acid profile. Bacteria involved in biohydrogenation belong to the Butyrivibrio group. This study was aimed at relating accumulation of biohydrogenation intermediates with shifts in Butyrivib...

Lipid metabolism in the rumen is responsible for the complex fatty acid profile of rumen outflow compared with the dietary fatty acid composition, contributing to the lipid profile of ruminant products. A method for the detailed dimethylacetal and fatty acid analysis of rumen contents was developed and applied to rumen content collected from lambs fed lucerne or concentrate based diets suppleme...

Food products from ruminants are the major dietary source of conjugated linoleic acids (CLA) for humans. The uniqueness of CLA in ruminant fat relates to the biohydrogenation of dietary unsaturated fatty acids by rumen bacteria. The CLA are intermediates in the biohydrogenation, and a portion escape the rumen and are incorporated into milk fat and body fat. In addition, the animal itself synthe...

Four different plant secondary metabolites were screened for their effect on rumen biohydrogenation of forage long-chain fatty acids, using dual-flow continuous culture fermenters. Treatments were as follows: control (no additive), positive control (12 mg/L of monensin), and plant extracts (500 and 1,000 mg/L of triterpene saponin; 250 and 500 mg/L of quercetin; 250 mg/L of eugenol; 500 mg/L of...

The effect of different pH values on rumen lipolysis and biohydrogenation was investigated during incubations of the rumen contents with 40 or 80 mg of soybean oil as the sole substrate. Mean pH values studied were 6.8, 6.3, 6.0, 5.6 and 5.2. Lipolysis was calculated from the decrease in fatty acids present in triacylglycerols (TAG), as well as from the accumulation of free fatty acids (FFA) du...

Conjugated linoleic acid (CLA) is presently under extensive research because of its potent anticarcinogenic effects and also because of its effects on the immune system and on lipid metabolism. The biological effects of these acids are briefly reviewed. Ruminant edible products are the richest natural sources of CLA because they are supposed to be mainly derived from rumen biohydrogenation of l...

From the simultaneous accumulation of hydrogenation intermediates and the disappearance of Isotricha prostoma after algae supplementation, we suggested a role of this ciliate and/or its associated bacteria in rumen biohydrogenation of unsaturated fatty acids. The experiments described here evaluated the role of I. prostoma and/or its associated endogenous and exogenous bacteria in rumen biohydr...

Developing novel strategies for improving the fatty acid composition of ruminant products relies upon increasing our understanding of rumen bacterial lipid metabolism. This study investigated whether flax or echium oil supplementation of steer diets could alter the rumen fatty acids and change the microbiome. Six Hereford × Friesian steers were offered grass silage/sugar beet pulp only (GS), or...