This study was conducted to investigate the effect of six cryoprotectants (dimethyl sulfoxide (DMSO), glycerol (Gly), methanol (MeOH), ethylene glycol (EG), 1,2-propylene glycol (PG) and N,N-dimethylformamide (DMF) on the survival of medaka (Oryzias lapites) embryos at low temperatures (0 and -5C). Firstly, the embryos at 8 to 16-cell stages were exposed to different concentrations (1 to 4 mol ...

Three species of flies were examined for chilling tolerance from the end of the pupariation to the onset of adult eclosion for the purpose of determining applicability for cold storage. For the housefly, Musca domestica (Diptera, Muscidae), survival to eclosion was affected by developmental stage/age, length of storage, and storage temperature. Houseflies that were closest to having experienced...

Models of long-term estrogen-deprived breast cancer cells are utilized in the laboratory to mimic clinical aromatase inhibitor-resistant breast cancer and serve as a tool to discover new therapeutic strategies. The MCF-7:5C and MCF-7:2A subclones were generated through long-term estrogen deprivation of estrogen receptor (ER)-positive MCF-7 cells, and represent anti-hormone‑resistant breast canc...

glycine betaine (gb) is one of the osmotic plant-protecting members that accumulate in response to abiotic stresses. an experiment was, therefore, conducted to test whether gb application at various concentrations (0, 10, 20 and 30 mm) through seed soaking or foliar spray methods would protect cucumber seedlings, subjected to chilling stress. at two true leave stages, after gb treatment, the se...

Excised shoot tips of sweet potato (Ipomoea babatas L.) were incubated in H2O2 or NaCl aqueous solution for 24h or 48h prior to a 3-day chilling at 2.5°C. Severity of chilling injury was visually observed during a post-chilling 7-day recovery at 21°C, and scored at 0 to 5 (none to most severe injury). In the first experiment, when cv. Purple (PUR) sweet potato shoots were subjected to 3-day chi...

the effects of coatingtreatments (waxand plasticbags) combined with methylsalicylate on the fruit quality and chilling injury of moro blood orange were studied during storage. treated fruits were kept in storage for 80 days at 5 oc and 90-95% relative humidity. some characteristics such as pulp and skin total phenolics, antioxidant capacity, total anthocyanin, lipid peroxidation, and the chilli...

the effects of different concentrations of salicylic acid as compared with control and as well with distilled water treatment were assessed on induction of resistance to chilling injury and quality of sweet lemon during its storage. for the purpose, sweet lemon fruits were treated by immersion method for 5 minutes in salicylic acid (200,400 and 600 mg/lit) and then packed in polyethylene bags t...

to study the effects of chilling on dormancy breaking and the increased germination characteristics of nepeta (germination percent and speed, seedling length, vigor index, wet and dry weight), a factorial experiment in a completely randomized design with 4 replications was implemented in a laboratory in 2014. factor a was the chilling treatment in three levels (control, one-month and two-month ...