The functional integrity of spermatozoa in various domestic animals like horse, cattle and swine is evaluated by hypo osmotic swelling test. The hypo osmotic swelling test has not been tested in the fresh semen of Nili-Ravi buffalo bull. The aim of the present study was evaluation of membrane integrity by establishing the best hypo osmotic swelling solution. Ten solutions with different osmolar...

This study evaluated night and day semen collection regimes in Holstein and Brahman bulls (four bulls of each breed) that were collected weekly, each during a morning and a night collection. Ejaculates (n=64) were obtained via artificial vagina over 4 weeks. The first collection of each week alternated between night and day. Two collection teams were employed. Bull behavior parameters included ...

Aim: Present investigation was carried out to study the physical characteristics of Kankrej bulls semen by evaluation of various semen parameters from neat semen and at various stages of semen preservation. Materials and Methods: A total of 60 ejaculates, 10 each from 6 mature Kankrej bulls, once in a week for 10 weeks, were collected and analyzed for various semen attributes. Result: The mean ...

The aim of current study was to investigate the changes in the quality parameters of Holstein bull semen during the heat stress and the effect of feeding a source of omega-3 fatty acids in this period. Samples were obtained from 19 Holstein bulls during the expected time of heat stress in Iran (June to September 2009). Control group (n=10) were fed a standard concentrate feed while treatment gr...

The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg mL-1 sodium selenite and the sperm motility and...

AIM The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. MATERIALS AND METHODS A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender whic...

antioxidant effect of olive leaf extract (ole) was studied on motility, viability, plasma membrane integrity of spermatozoa and malondialdehyde production in 12 ross 308 roosters at their 30 weeks of age. semen samples were collected by abdominal massage in 5 times. in each session after the initial sperm assessment, collected samples were pooled and diluted with sexton extender.samples were sp...

The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three ejaculates per bull were evaluated. Frozen semen samples were thawed and the acrosomal region of sperm cells was evaluated by transmission electron microscopy (TEM) before ...

the aim of this study was to investigate the effect of adding different levels of zinc sulfate in semen extender on bovine sperm quality during cryopreservation. semen was collected from 4 holstein bull twice a week and the proper samples were pooled. semen were allocated to four extender treatments consisted of 0, 50, 100, 150 µm of zinc sulfate in 5 replicates and then, were frozen. post-thaw...